Detection of Epstein-Barr virus by polymerase chain reaction

Détails

ID Serval
serval:BIB_D3D0D140CD9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Detection of Epstein-Barr virus by polymerase chain reaction
Périodique
Journal of Clinical Microbiology
Auteur⸱e⸱s
Telenti  A., Marshall  W. F., Smith  T. F.
ISSN
0095-1137 (Print)
Statut éditorial
Publié
Date de publication
10/1990
Volume
28
Numéro
10
Pages
2187-90
Notes
Journal Article --- Old month value: Oct
Résumé
The polymerase chain reaction (PCR) was used to study DNA extracted from the blood of 25 transplant patients, 5 patients with infectious mononucleosis, and 13 healthy subjects and autopsy or biopsy tissue from 29 patients with lymphoproliferative disorders. Primers were directed to conserved regions of the Epstein-Barr virus (EBV) genome encoding capsid protein gp220 and Epstein-Barr nuclear antigen 1. Specific EBV amplification was found in the blood of 11 of 25 transplant patients and all patients with infectious mononucleosis. All patients with lymphoproliferative disorders occurring in the presence of immunosuppression (eight organ transplant patients and two patients with acquired immunodeficiency syndrome) had biopsies positive for EBV by PCR. Only 1 of 19 samples from lymphomas or leukemias unrelated to immunosuppression contained EBV. PCR confirmed the very close association of EBV and lymphoproliferative disorders occurring in the presence of immunosuppression. The significance of detecting EBV sequences in the blood of transplant patients, particularly in relationship to lymphoproliferation, requires further study.
Mots-clé
Adult Base Sequence DNA Probes DNA, Viral/blood/genetics/isolation & purification Herpesvirus 4, Human/genetics/*isolation & purification Humans Immune Tolerance Leukemia/microbiology Lymphoproliferative Disorders/microbiology Molecular Sequence Data Organ Transplantation Polymerase Chain Reaction/*methods
Pubmed
Web of science
Création de la notice
25/01/2008 14:46
Dernière modification de la notice
20/08/2019 15:53
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