Lactate is a preferential oxidative energy substrate over glucose for neurons in culture
Détails
ID Serval
serval:BIB_D34AA7B3AEF4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lactate is a preferential oxidative energy substrate over glucose for neurons in culture
Périodique
Journal of Cerebral Blood Flow and Metabolism
ISSN
0271-678X (Print)
Statut éditorial
Publié
Date de publication
11/2003
Volume
23
Numéro
11
Pages
1298-306
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
The authors investigated concomitant lactate and glucose metabolism in primary neuronal cultures using 13C- and 1H-NMR spectroscopy. Neurons were incubated in a medium containing either [1-13C]glucose and different unlabeled lactate concentrations, or unlabeled glucose and different [3-13C]lactate concentrations. Overall, 13C-NMR spectra of cellular extracts showed that more 13C was incorporated into glutamate when lactate was the enriched substrate. Glutamate 13C-enrichment was also found to be much higher in lactate-labeled than in glucose-labeled conditions. When glucose and lactate concentrations were identical (5.5 mmol/L), relative contributions of glucose and lactate to neuronal oxidative metabolism amounted to 21% and 79%, respectively. Results clearly indicate that when neurons are in the presence of both glucose and lactate, they preferentially use lactate as their main oxidative substrate.
Mots-clé
Animals
Animals, Newborn
Carbon Isotopes
Cells, Cultured
Citric Acid Cycle
*Energy Metabolism
Glucose/*metabolism
Glutamic Acid/metabolism
Lactates/*metabolism
Neurons/*metabolism
Nuclear Magnetic Resonance, Biomolecular
Oxidative Phosphorylation
Rats
Rats, Wistar
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:17
Dernière modification de la notice
20/08/2019 16:53