Fibrinmatrix erhöht Adhärenz von regenerativen peripheren Nervenzellen [Fibrin matrix enhances adherence of peripheral nerve regenerative cells]

Détails

ID Serval
serval:BIB_D3398D99E6D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fibrinmatrix erhöht Adhärenz von regenerativen peripheren Nervenzellen [Fibrin matrix enhances adherence of peripheral nerve regenerative cells]
Périodique
Handchirurgie, Mikrochirurgie, plastische Chirurgie
Auteur⸱e⸱s
Kalbermatten D.F., Kingham P.J., Mahay D., Balcin H., Pierer G., Terenghi G.
ISSN
0722-1819
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
40
Numéro
2
Pages
75-80
Langue
allemand
Résumé
Optimal seeding of a nerve conduit with cells is a core problem in tissue engineering of constructing an artificial nerve substitute to gap lesions in the peripheral nerve system. An ideal nerve gap substitute would have to present an equally distributed number of cells that can activate the regrowing axons. This work shows a new in vitro technique of two-step seeding of cells inside a conduit and on layered mats that allows a valuable targeting of the cells and a proven survival in the environment of poly-3-hydroxybutyrate (PHB) conduits. The technique uses two components of diluted fibrin glue Tisseel. Initially, the chosen area on the mat was coated with thrombin followed from the seeding of a fibrinogen-cell compound. Using Sprague Dawley rat cells, we could demonstrate with immunohistochemistry (S100, DAPI) techniques that undifferentiated (uMSC) and Schwann cells (SC) mimicking differentiated mesenchymal stem cells (dMSC) as well as SC can be suspended and targeted significantly better in dissolvable diluted fibrin glue than in growth medium. Analysis showed significantly better values for adherence (p < 0.001) and drop off (p < 0.05) from seeded cells. Using this two-step application allows the seeding of the cells to be more precise and simplifies the handling of cell transplantation.
Mots-clé
Animals, Cell Count, Cell Culture Techniques, Cell Differentiation, Cell Transplantation, Fibrin Tissue Adhesive, Hydroxybutyrates, Immunohistochemistry, Microscopy, Nerve Regeneration, Peripheral Nerves, Polyesters, Rats, Rats, Sprague-Dawley, Schwann Cells, Time Factors, Tissue Engineering
Pubmed
Web of science
Création de la notice
13/03/2009 17:08
Dernière modification de la notice
20/08/2019 15:53
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