Polyesterurethane foam scaffold for smooth muscle cell tissue engineering

Détails

ID Serval
serval:BIB_D271C806EB9B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Polyesterurethane foam scaffold for smooth muscle cell tissue engineering
Périodique
Biomaterials
Auteur(s)
Danielsson  C., Ruault  S., Simonet  M., Neuenschwander  P., Frey  P.
ISSN
0142-9612
Statut éditorial
Publié
Date de publication
03/2006
Peer-reviewed
Oui
Volume
27
Numéro
8
Pages
1410-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
Reconstruction of the genitourinary tract, using engineered urological tissues, requires a mechanically stable biodegradable and biocompatible scaffold and cultured cells. Such engineered autologous tissue would have many clinical implications. In this study a highly porous biodegradable polyesterurethane-foam, DegraPol was evaluated with tissue engineered human primary bladder smooth muscle cells. The cell-polymer constructs were characterized by histology, scanning electron microscopy, immunohistochemistry and proliferation assays. Smooth muscle cells grown on DegraPol showed the same morphology as when grown on control polystyrene surface. Positive immunostaining with alpha smooth muscle actin indicated the preservation of the specific cell phenotype. Micrographs from scanning electron microscopy showed that the cells grew on the foam surface as well as inside the pores. In addition they grew as cell aggregates within the foam. The smooth muscle cells proliferated on the Degrapol; however, proliferation rate decreased due to apoptosis with time in culture. This study showed that Degrapol has the potential to be used as a scaffold.
Mots-clé
Apoptosis/physiology *Biocompatible Materials Cell Adhesion/physiology Cell Proliferation Cells, Cultured Child Humans Immunohistochemistry *Myocytes, Smooth Muscle/cytology/physiology/ultrastructure *Polyesters *Polyurethanes *Tissue Engineering
Pubmed
Web of science
Création de la notice
28/02/2008 11:25
Dernière modification de la notice
20/08/2019 16:52
Données d'usage