MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens

Détails

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Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_D22649B3951C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens
Périodique
Nucleic Acids Research
Auteur(s)
Trouiller B., Schaefer D.G., Charlot F., Nogué F.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2006
Volume
34
Numéro
1
Pages
232-242
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
MSH2 is a central component of the mismatch repair pathway that targets mismatches arising during DNA replication, homologous recombination (HR) and in response to genotoxic stresses. Here, we describe the function of MSH2 in the moss Physcomitrella patens, as deciphered by the analysis of loss of function mutants. Ppmsh2 mutants display pleiotropic growth and developmental defects, which reflect genomic instability. Based on loss of function of the APT gene, we estimated this mutator phenotype to be at least 130 times higher in the mutants than in wild type. We also found that MSH2 is involved in some but not all the moss responses to genotoxic stresses we tested. Indeed, the Ppmsh2 mutants were more tolerant to cisplatin and show higher sensitivity to UV-B radiations. PpMSH2 gene involvement in HR was studied by assessing gene targeting (GT) efficiency with homologous and homeologous sequences. GT efficiency with homologous sequences was slightly decreased in the Ppmsh2 mutant compared with wild type. Strikingly GT efficiency with homeologous sequences decreased proportionally to sequence divergence in the wild type whereas it remained unaffected in the mutants. Those results demonstrate the role of PpMSH2 in the maintenance of genome integrity and in homologous and homeologous recombination.
Mots-clé
Bryopsida/genetics, Bryopsida/growth & development, Bryopsida/radiation effects, MutS Homolog 2 Protein/genetics, MutS Homolog 2 Protein/physiology, Mutagens/toxicity, Plant Proteins/genetics, Plant Proteins/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/07/2018 9:00
Dernière modification de la notice
20/08/2019 15:52
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