Molecular recognition of B-DNA by Hoechst 33258.

Détails

ID Serval
serval:BIB_D18EF881F3E3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular recognition of B-DNA by Hoechst 33258.
Périodique
Nucleic Acids Research
Auteur(s)
Harshman K.D., Dervan P.B.
ISSN
0305-1048
Statut éditorial
Publié
Date de publication
1985
Peer-reviewed
Oui
Volume
13
Numéro
13
Pages
4825-4835
Langue
anglais
Résumé
The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl-EDTA X Fe(II) [MPE X Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five +/- one bp in size and rich in A X T DNA base pairs. The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. Hydrophobic interaction of the flanking phenol and N-methylpiperazine rings would afford a steric blockade of one additional base pair on each side.
Mots-clé
Base Sequence, Benzimidazoles/pharmacology, Binding Sites, Bisbenzimidazole/pharmacology, DNA, Distamycins/pharmacology, Netropsin/pharmacology
Pubmed
Web of science
Création de la notice
12/03/2008 12:36
Dernière modification de la notice
20/08/2019 15:51
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