Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration.

Détails

ID Serval
serval:BIB_D1658F6AAE62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration.
Périodique
Cell Death and Differentiation
Auteur⸱e⸱s
Pétrilli V., Papin S., Dostert C., Mayor A., Martinon F., Tschopp J.
ISSN
1350-9047 (Print)
ISSN-L
1350-9047
Statut éditorial
Publié
Date de publication
2007
Volume
14
Numéro
9
Pages
1583-1589
Langue
anglais
Résumé
Inflammasomes are Nod-like receptor(NLR)- and caspase-1-containing cytoplasmic multiprotein complexes, which upon their assembly, process and activate the proinflammatory cytokines interleukin (IL)-1beta and IL-18. The inflammasomes harboring the NLR members NALP1, NALP3 and IPAF have been best characterized. While the IPAF inflammasome is activated by bacterial flagellin, activation of the NALP3 inflammasome is triggered not only by several microbial components, but also by a plethora of danger-associated host molecules such as uric acid. How NALP3 senses these chemically unrelated activators is not known. Here, we provide evidence that activation of NALP3, but not of the IPAF inflammasome, is blocked by inhibiting K(+) efflux from cells. Low intracellular K(+) is also a requirement for NALP1 inflammasome activation by lethal toxin of Bacillus anthracis. In vitro, NALP inflammasome assembly and caspase-1 recruitment occurs spontaneously at K(+) concentrations below 90 mM, but is prevented at higher concentrations. Thus, low intracellular K(+) may be the least common trigger of NALP-inflammasome activation.
Mots-clé
Animals, Apoptosis Regulatory Proteins/metabolism, CARD Signaling Adaptor Proteins/metabolism, Calcium-Binding Proteins/metabolism, Carrier Proteins/metabolism, Caspase 1/metabolism, Cell Line, Humans, Inflammation/metabolism, Interleukin-18/metabolism, Interleukin-1beta/metabolism, Macrophages, Peritoneal/metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Potassium/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:18
Dernière modification de la notice
20/08/2019 15:51
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