Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes

Détails

ID Serval
serval:BIB_D0E1157D72BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes
Périodique
Journal of Immunology
Auteur(s)
Carrel  S., Gross  N., Salvi  S.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
05/1978
Peer-reviewed
Oui
Volume
120
Numéro
5
Pages
1587-93
Notes
Journal Article --- Old month value: May
Résumé
An antiserum was produced by immunization of rabbits with membrane preparations of a human lymphoblastoid B cell line, Daudi. After absorption with a human endometrial carcinoma cell line, this antiserum appeared to be specific for antigen(s) present on adult and fetal thymocytes as well as on tonsillar lymphocytes but absent, or present in very small amounts, on normal or phytohemagglutinin- (PHA) stimulated peripheral blood lymphocytes (PBL). When T and B cell-enriched fractions from tonsillar lymphocytes were tested with the anti-Daudi serum, the reactivity was equally distributed in each population. Among 13 human lymphoblastoid cell lines tested, reactivity was demonstrated on three out of four T cell lines, and on four out of nine B cell lines. The positive reacting B cell lines were derived from two African and two American Burkitt lymphomas. The antigen(s) described does not seem to be related either to human Ia-type antigens or to Epstein-Barr virus-associated antigens because these antigens are not present on fetal or adult thymocytes. Reciprocal absorption experiments indicate that this anti-Daudi serum detects the same antigenic structures present on certain subpopulations of T and B lymphocytes.
Mots-clé
Absorption Animals *Antigens Antilymphocyte Serum/*pharmacology Cell Line Cell Membrane/immunology Cell Separation Cytotoxicity, Immunologic Humans Immunoglobulin M Lectins/pharmacology Lymphocytes/*immunology Rabbits T-Lymphocytes/immunology Tonsil/immunology
Pubmed
Web of science
Création de la notice
20/01/2008 15:56
Dernière modification de la notice
20/08/2019 15:51
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