Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea.

Détails

ID Serval
serval:BIB_D0B32AB307C1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea.
Périodique
Journal of virology
Auteur⸱e⸱s
Ruggeri F.M., Johansen K., Basile G., Kraehenbuhl J.P., Svensson L.
ISSN
0022-538X
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
72
Numéro
4
Pages
2708-14
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Rotaviruses are the major cause of severe diarrhea in infants and young children worldwide. Due to their restricted site of replication, i.e., mature enterocytes, local intestinal antibodies have been proposed to play a major role in protective immunity. Whether secretory immunoglobulin A (IgA) antibodies alone can provide protection against rotavirus diarrhea has not been fully established. To address this question, a library of IgA monoclonal antibodies (MAbs) previously developed against different proteins of rhesus rotavirus was used. A murine hybridoma "backpack tumor" model was established to examine if a single MAb secreted onto mucosal surfaces via the normal epithelial transport pathway was capable of protecting mice against diarrhea upon oral challenge with rotavirus. Of several IgA and IgG MAbs directed against VP8 and VP6 of rotavirus, only IgA VP8 MAbs (four of four) were found to protect newborn mice from diarrhea. An IgG MAb recognizing the same epitope as one of the IgA MAbs tested failed to protect mice from diarrhea. We also investigated if antibodies could be transcytosed in a biologically active form from the basolateral domain to the apical domain through filter-grown Madin-Darby canine kidney (MDCK) cells expressing the polymeric immunoglobulin receptor. Only IgA antibodies with VP8 specificity (four of four) neutralized apically administered virus. The results support the hypothesis that secretory IgA antibodies play a major role in preventing rotavirus diarrhea. Furthermore, the results show that the in vivo and in vitro methods described are useful tools for exploring the mechanisms of viral mucosal immunity.
Mots-clé
Animals, Animals, Newborn, Antibodies, Viral, Biological Transport, Cell Line, Diarrhea, Dogs, Epithelial Cells, Immunoglobulin A, Secretory, Immunoglobulin G, Intestines, Mice, Mice, Inbred BALB C, Neutralization Tests, RNA-Binding Proteins, Receptors, Polymeric Immunoglobulin, Rotavirus, Rotavirus Infections, Tumor Cells, Cultured, Viral Nonstructural Proteins
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 16:50
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