The generation and utilization of a cancer-oriented representation of the human transcriptome by using expressed sequence tags.

Détails

ID Serval
serval:BIB_D06F4D273E1F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The generation and utilization of a cancer-oriented representation of the human transcriptome by using expressed sequence tags.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Brentani H., Caballero O.L., Camargo A.A., da Silva A.M., da Silva W.A., Dias Neto E., Grivet M., Gruber A., Guimaraes P.E., Hide W., Iseli C., Jongeneel C.V., Kelso J., Nagai M.A., Ojopi E.P., Osorio E.C., Reis E.M., Riggins G.J., Simpson A.J., de Souza S., Stevenson B.J., Strausberg R.L., Tajara E.H., Verjovski-Almeida S., Acencio M.L., Bengtson M.H., Bettoni F., Bodmer W.F., Briones M.R., Camargo L.P., Cavenee W., Cerutti J.M., Coelho Andrade L.E., Costa dos Santos P.C., Ramos Costa M.C., da Silva I.T., Estécio M.R. , Sa Ferreira K., Furnari F.B., Faria M., Galante P.A., Guimaraes G.S., Holanda A.J., Kimura E.T., Leerkes M.R., Lu X., Maciel R.M., Martins E.A., Massirer K.B., Melo A.S., Mestriner C.A., Miracca E.C., Miranda L.L., Nobrega F.G., Oliveira P.S., Paquola A.C., Pandolfi J.R., Campos Pardini M.I., Passetti F., Quackenbush J., Schnabel B., Sogayar M.C., Souza J.E., Valentini S.R., Zaiats A.C., Amaral E.J., Arnaldi L.A., de Araújo A.G., de Bessa S.A., Bicknell D.C., Ribeiro de Camaro M.E., Carraro D.M., Carrer H., Carvalho A.F., Colin C., Costa F., Curcio C., Guerreiro da Silva I.D., Pereira da Silva N., Dellamano M., El-Dorry H., Espreafico E.M., Scattone Ferreira A.J., Ayres Ferreira C., Fortes M.A., Gama A.H., Giannella-Neto D., Giannella M.L., Giorgi R.R., Goldman G.H., Goldman M.H., Hackel C., Ho P.L., Kimura E.M., Kowalski L.P., Krieger J.E., Leite L.C., Lopes A., Luna A.M., Mackay A., Mari S.K., Marques A.A., Martins W.K., Montagnini A., Mourão Neto M. , Nascimento A.L., Neville A.M., Nobrega M.P., O'Hare M.J., Otsuka A.Y., Ruas de Melo A.I., Paco-Larson M.L., Guimarães Pereira G. , Pereira da Silva N., Pesquero J.B., Pessoa J.G., Rahal P., Rainho C.A., Rodrigues V., Rogatto S.R., Romano C.M., Romeiro J.G., Rossi B.M., Rusticci M., Guerra de Sá R. , Sant' Anna S.C., Sarmazo M.L., Silva T.C., Soares F.A., Sonati Mde F, de Freitas Sousa J., Queiroz D., Valente V., Vettore A.L., Villanova F.E., Zago M.A., Zalcberg H.
Collaborateur⸱rice⸱s
Human Cancer Genome Project/Cancer Genome Anatomy Project Annotation Consortium, Human Cancer Genome Project Sequencing Consortium
ISSN
0027-8424[print], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
100
Numéro
23
Pages
13418-13423
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Whereas genome sequencing defines the genetic potential of an organism, transcript sequencing defines the utilization of this potential and links the genome with most areas of biology. To exploit the information within the human genome in the fight against cancer, we have deposited some two million expressed sequence tags (ESTs) from human tumors and their corresponding normal tissues in the public databases. The data currently define approximately 23,500 genes, of which only approximately 1,250 are still represented only by ESTs. Examination of the EST coverage of known cancer-related (CR) genes reveals that <1% do not have corresponding ESTs, indicating that the representation of genes associated with commonly studied tumors is high. The careful recording of the origin of all ESTs we have produced has enabled detailed definition of where the genes they represent are expressed in the human body. More than 100,000 ESTs are available for seven tissues, indicating a surprising variability of gene usage that has led to the discovery of a significant number of genes with restricted expression, and that may thus be therapeutically useful. The ESTs also reveal novel nonsynonymous germline variants (although the one-pass nature of the data necessitates careful validation) and many alternatively spliced transcripts. Although widely exploited by the scientific community, vindicating our totally open source policy, the EST data generated still provide extensive information that remains to be systematically explored, and that may further facilitate progress toward both the understanding and treatment of human cancers.
Mots-clé
Chromosome Mapping, Databases, Genetic, Expressed Sequence Tags, Gene Expression Regulation, Neoplastic, Genetic Variation, Humans, Neoplasms/genetics, Neoplasms/metabolism, Polymorphism, Single Nucleotide, Proteome, RNA, Messenger/metabolism, Tissue Distribution
Pubmed
Web of science
Création de la notice
24/01/2008 15:39
Dernière modification de la notice
20/08/2019 15:50
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