On-line desorption of dried blood spot: A novel approach for the direct LC/MS analysis of micro-whole blood samples.

Détails

ID Serval
serval:BIB_D0236DB53B01
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
On-line desorption of dried blood spot: A novel approach for the direct LC/MS analysis of micro-whole blood samples.
Périodique
Journal of pharmaceutical and biomedical analysis
Auteur(s)
Déglon J., Thomas A., Cataldo A., Mangin P., Staub C.
ISSN
1873-264X[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
49
Numéro
4
Pages
1034-9
Langue
anglais
Résumé
The aim of this work is to present a new concept, called on-line desorption of dried blood spots (on-line DBS), allowing the direct analysis of a dried blood spot coupled to liquid chromatography mass spectrometry device (LC/MS). The system is based on an inox cell which can receive a blood sample (10 microL) previously spotted on a filter paper. The cell is then integrated into LC/MS system where the analytes are desorbed out of the paper towards a column switching system ensuring the purification and separation of the compounds before their detection on a single quadrupole MS coupled to atmospheric pressure chemical ionisation (APCI) source. The described procedure implies that no pretreatment is necessary in spite the analysis is based on whole blood sample. To ensure the applicability of the concept, saquinavir, imipramine, and verapamil were chosen. Despite the use of a small sampling volume and a single quadrupole detector, on-line DBS allowed the analyses of these three compounds over their therapeutic concentrations from 50 to 500 ng/mL for imipramine and verapamil and from 100 to 1000 ng/mL for saquinavir. Moreover, the method showed good repeatability with relative standard deviation (RSD) lower than 15% based on two levels of concentration (low and high). Function responses were found to be linear over the therapeutic concentration for each compound and were used to determine the concentrations of real patient samples for saquinavir. Comparison of the founded values with those of a validated method used routinely in a reference laboratory showed a good correlation between the two methods. Moreover, good selectivity was observed ensuring that no endogenous or chemical components interfered with the quantitation of the analytes. This work demonstrates the feasibility and applicability of the on-line DBS procedure for bioanalysis.
Mots-clé
Adsorption, Anti-HIV Agents/blood, Blood Chemical Analysis/methods, Calcium Channel Blockers/blood, Chromatography, High Pressure Liquid, Humans, Mass Spectrometry, Online Systems, Reference Standards, Reproducibility of Results, Saquinavir/blood, Solvents, Verapamil/blood
Pubmed
Web of science
Création de la notice
15/01/2010 16:41
Dernière modification de la notice
20/08/2019 15:50
Données d'usage