CCL8 and the Immune Control of Cytomegalovirus in Organ Transplant Recipients.

Détails

ID Serval
serval:BIB_CFBFAC10D4D2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CCL8 and the Immune Control of Cytomegalovirus in Organ Transplant Recipients.
Périodique
American Journal of Transplantation
Auteur⸱e⸱s
Lisboa L.F., Egli A., Fairbanks J., O'Shea D., Manuel O., Husain S., Kumar D., Humar A.
ISSN
1600-6143 (Electronic)
ISSN-L
1600-6135
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
15
Numéro
7
Pages
1882-1892
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Monitoring of cytomegalovirus cell-mediated immunity is a promising tool for the refinement of preventative and therapeutic strategies posttransplantation. Typically, the interferon-γ response to T cell stimulation is measured. We evaluated a broad range of cytokine and chemokines to better characterize the ex vivo host-response to CMV peptide stimulation. In a cohort of CMV viremic organ transplant recipients, chemokine expression-specifically CCL8 (AUC 0.849 95% CI 0.721-0.978; p = 0.003) and CXCL10 (AUC 0.841, 95% CI 0.707-0.974; p = 0.004)-was associated with control of viral replication. In a second cohort of transplant recipients at high-risk for CMV, the presence of a polymorphism in the CCL8 promoter conferred an increased risk of viral replication after discontinuation of antiviral prophylaxis (logrank hazard ratio 3.6; 95% CI 2.077-51.88). Using cell-sorting experiments, we determined that the primary cell type producing CCL8 in response to CMV peptide stimulation was the monocyte fraction. Finally, in vitro experiments using standard immunosuppressive agents demonstrated a dose-dependent reduction in CCL8 production. Chemokines appear to be important elements of the cell-mediated response to CMV infection posttransplant, as here suggested for CCL8, and translation of this knowledge may allow for the tailoring and improvement of preventative strategies.
Mots-clé
Antiviral Agents/therapeutic use, Case-Control Studies, Chemokine CCL8/genetics, Chemokine CCL8/metabolism, Cohort Studies, Cytokines/genetics, Cytokines/metabolism, Cytomegalovirus/isolation & purification, Cytomegalovirus Infections/immunology, Cytomegalovirus Infections/metabolism, Follow-Up Studies, Genes, MHC Class I/immunology, Graft Rejection, Graft Survival, Humans, Immunity, Cellular, Immunosuppression, Organ Transplantation, Peptide Fragments/pharmacology, Polymorphism, Single Nucleotide/genetics, Postoperative Complications, Prognosis, Promoter Regions, Genetic, Risk Factors, Survival Rate, T-Lymphocytes/immunology, Transplant Recipients, Viremia/epidemiology, Viremia/immunology, Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/07/2015 12:13
Dernière modification de la notice
20/08/2019 16:50
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