Preliminary evaluation of two new rapid immunoturbidimetric D-dimer assays in patients with clinically suspected venous thromboembolism (VTE).

Détails

ID Serval
serval:BIB_CEE7DD2C834A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Preliminary evaluation of two new rapid immunoturbidimetric D-dimer assays in patients with clinically suspected venous thromboembolism (VTE).
Périodique
Thrombosis and Haemostasis
Auteur(s)
Houbouyan-Reveillard L.L., Mihoubi A., Houdijk W.P., Qanadli S., Joseph T., Courret J.P., Page B., Goguel A.F.
ISSN
0340-6245
Statut éditorial
Publié
Date de publication
11/2000
Peer-reviewed
Oui
Volume
84
Numéro
5
Pages
770-4
Langue
anglais
Notes
Publication types: Clinical Trial ; Comparative Study ; Journal Article ; Randomized Controlled Trial
Résumé
The practical utility and diagnostic accuracy of two new rapid, automated and quantitative immunoturbidimetric D-dimer methods have been evaluated in a population of 123 randomly selected patients with suspected VTE. The STA Liatest D-dimer and MDA D-dimer methods are based on the photo-optical measurement of the rate of agglutination of antibody-coated latex particles. The VIDAS D-dimer automated Elisa was used as the reference method. Diagnosis was confirmed in 51 patients (29 PE, 19 DVT, 3 DVT+PE). The immunoturbidimetric methods compared favorably with the VIDAS Elisa as judged from the correlation coefficients of linear regression lines (r = 0.82, MDA vs VIDAS; r = 0.75, STA vs VIDAS) and areas under the curve of ROC plots (VIDAS 0.83; STA 0.83; MDA 0.81). At a discriminant value of 500 ng/mL, all three D-dimer assays showed high sensitivity (96-98%), high NPV (93-97%) and moderate specificity (39-42%). Reproducibility of results around the cut-off is an important aspect of the diagnostic utility of D-dimer assays. CV's of duplicate determinations in this critical zone showed average values of 5.4% and 17.0% for MDA and STA, respectively. These data demonstrate that such rapid and automated latex-based methods for the quantitative measurement of D-dimer hold promise as reliable and cost-efficient approaches for the exclusion of VTE. Prospective patient management studies will be required to confirm this.
Mots-clé
Adult, Aged, Aged, 80 and over, Fibrin Fibrinogen Degradation Products/analysis, Humans, Immunoassay/methods, Middle Aged, Sensitivity and Specificity, Venous Thrombosis/blood, Venous Thrombosis/diagnosis
Pubmed
Web of science
Création de la notice
09/04/2008 17:12
Dernière modification de la notice
20/08/2019 16:49
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