Loop-mediated isothermal amplification (LAMP) as an alternative to PCR: A rapid on-site detection of gene doping.

Détails

ID Serval
serval:BIB_CEB715255F60
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Loop-mediated isothermal amplification (LAMP) as an alternative to PCR: A rapid on-site detection of gene doping.
Périodique
Drug testing and analysis
Auteur⸱e⸱s
Salamin O., Kuuranne T., Saugy M., Leuenberger N.
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Statut éditorial
Publié
Date de publication
11/2017
Peer-reviewed
Oui
Volume
9
Numéro
11-12
Pages
1731-1737
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Innovation in medical research has been diverted at multiple occasions to enhance human performance. The predicted great progress in gene therapy has raised some concerns regarding its misuse in the world of sports (gene doping) for several years now. Even though there is no evidence that gene doping has ever been used in sports, the continuous improvement of gene therapy techniques increases the likelihood of abuse. Therefore, since 2004, efforts have been invested by the anti-doping community and WADA for the development of detection methods. Several nested PCR and qPCR-based strategies exploiting the absence of introns in the transgenic DNA have been proposed for the long-term detection of transgene in blood. Despite their great sensitivity, those protocols are hampered by limitations of the techniques that can be cumbersome and costly. The purpose of this perspective is to describe a new approach based on loop-mediated isothermal amplification (LAMP) for the detection of gene doping. This protocol enables a rapid and simple method to amplify nucleic acids with a high sensitivity and specificity and with a simple visual detection of the results. LAMP is already being used in clinical application for the detection of viruses or mutations. Therefore, this technique has the potential to be further developed for the detection of foreign genetic material in elite athletes. Copyright © 2017 John Wiley & Sons, Ltd.

Mots-clé
LAMP, PCR, gene doping
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/10/2017 17:15
Dernière modification de la notice
20/08/2019 16:49
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