Cellular source and mechanisms of high transcriptome complexity in the Mammalian testis.

Détails

Ressource 1Télécharger: mmc3.pdf (4989.81 [Ko])
Etat: Public
Version: Final published version
Document(s) secondaire(s)
Télécharger: mmc1 (18).pdf (91.47 [Ko])
Etat: Public
Version: Supplementary document
Télécharger: mmc2.xlsx (25.40 [Ko])
Etat: Public
Version: Supplementary document
ID Serval
serval:BIB_CE7101CCD7BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cellular source and mechanisms of high transcriptome complexity in the Mammalian testis.
Périodique
Cell Reports
Auteur(s)
Soumillon M., Necsulea A., Weier M., Brawand D., Zhang X., Gu H., Barthès P., Kokkinaki M., Nef S., Gnirke A., Dym M., de Massy B., Mikkelsen T.S., Kaessmann H.
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
2013
Volume
3
Numéro
6
Pages
2179-2190
Langue
anglais
Résumé
Understanding the extent of genomic transcription and its functional relevance is a central goal in genomics research. However, detailed genome-wide investigations of transcriptome complexity in major mammalian organs have been scarce. Here, using extensive RNA-seq data, we show that transcription of the genome is substantially more widespread in the testis than in other organs across representative mammals. Furthermore, we reveal that meiotic spermatocytes and especially postmeiotic round spermatids have remarkably diverse transcriptomes, which explains the high transcriptome complexity of the testis as a whole. The widespread transcriptional activity in spermatocytes and spermatids encompasses protein-coding and long noncoding RNA genes but also poorly conserves intergenic sequences, suggesting that it may not be of immediate functional relevance. Rather, our analyses of genome-wide epigenetic data suggest that this prevalent transcription, which most likely promoted the birth of new genes during evolution, is facilitated by an overall permissive chromatin in these germ cells that results from extensive chromatin remodeling.
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/08/2013 8:51
Dernière modification de la notice
20/08/2019 16:49
Données d'usage