Intracellular calcium oscillations in astrocytes: a highly plastic, bidirectional form of communication between neurons and astrocytes in situ

Détails

ID Serval
serval:BIB_CE2B1BE0A698
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Intracellular calcium oscillations in astrocytes: a highly plastic, bidirectional form of communication between neurons and astrocytes in situ
Périodique
Journal of Neuroscience
Auteur⸱e⸱s
Pasti  L., Volterra  A., Pozzan  T., Carmignoto  G.
ISSN
0270-6474 (Print)
Statut éditorial
Publié
Date de publication
10/1997
Volume
17
Numéro
20
Pages
7817-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Résumé
The spatial-temporal characteristics of intracellular calcium ([Ca2+]i) changes elicited in neurons and astrocytes by various types of stimuli were investigated by means of confocal fluorescent microscopy in acute rat brain slices loaded with the Ca2+ indicator indo-1. Neurons and astrocytes from the visual cortex and CA1 hippocampal region were identified in situ on the basis of their morphological, electrophysiological, and pharmacological features. We show here that stimulation of neuronal afferents triggered periodic [Ca2+]i oscillations in astrocytes. The frequency of these oscillations was under a dynamic control by neuronal activity as it changed according to the pattern of stimulation. After repetitive episodes of neuronal stimulation as well as repetitive stimulation with a metabotropic glutamate receptor agonist, astrocytes displayed a long-lasting increase in [Ca2+]i oscillation frequency. Oscillating astrocytes were accompanied by repetitive [Ca2+]i elevations in adjacent neurons, most likely because of the release of glutamate via a tetanus toxin-resistant process. These results reveal that [Ca2+]i oscillations in astrocytes represent a highly plastic signaling system that underlies the reciprocal communication between neurons and astrocytes.
Mots-clé
Animals Astrocytes/metabolism/*physiology Calcium/*metabolism Cell Communication/*physiology Electric Stimulation Hippocampus/cytology/growth & development/metabolism Intracellular Membranes/*metabolism Neuronal Plasticity/*physiology Neurons/metabolism/*physiology Rats Rats, Wistar Receptors, Metabotropic Glutamate/physiology Visual Cortex/cytology/growth & development/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 14:37
Dernière modification de la notice
20/08/2019 15:48
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