Biosynthesis of the IgA antibody receptor: a model for the transepithelial sorting of a membrane glycoprotein

Détails

ID Serval
serval:BIB_CD96A791C9C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Biosynthesis of the IgA antibody receptor: a model for the transepithelial sorting of a membrane glycoprotein
Périodique
Cell
Auteur⸱e⸱s
Solari  R., Kraehenbuhl  J. P.
ISSN
0092-8674 (Print)
Statut éditorial
Publié
Date de publication
01/1984
Volume
36
Numéro
1
Pages
61-71
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
Secretory IgA dimer antibodies in exosecretions provide the primary immunological defense for mucosal surfaces. Transmission of IgA2 across the epithelia of mucous and exocrine glands is mediated by a receptor called secretory component (SC). Using three antibodies directed against different domains of SC, we examine its processing in the lactating rabbit mammary gland. SC is synthesized as a core glycosylated transmembrane glycoprotein on the rough endoplasmic reticulum. Pulse-chase experiments reveal the time course of SC maturation in the Golgi, as demonstrated by the acquisition of Endo H resistance (30-60 min). The subsequent routing of SC to the basolateral plasma membrane, where IgA2 binding and endocytosis occurs, the cleavage of the membrane anchoring domain of SC, and the exocytosis from the apical plasma membrane of IgA, bound to the ectoplasmic domain of SC takes place rapidly (30-60 min). Thus maturation in the Golgi may represent the rate limiting step in SC routing. We also demonstrate that SC exists in several conformational states that are processed at different rates.
Mots-clé
Animals Cell Membrane/metabolism Endoplasmic Reticulum/metabolism Female Glycoside Hydrolases/pharmacology Golgi Apparatus/metabolism Immunoglobulin A, Secretory/*metabolism Immunoglobulin Fragments/*biosynthesis Kinetics Lactation Mammary Glands, Animal/*metabolism Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Weight Pregnancy Protein Processing, Post-Translational Rabbits Secretory Component/*biosynthesis
Pubmed
Web of science
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 15:48
Données d'usage