Differential regulation of SAP8 and SAP9, which encode two new members of the secreted aspartic proteinase family in Candida albicans
Détails
ID Serval
serval:BIB_CD7472249223
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential regulation of SAP8 and SAP9, which encode two new members of the secreted aspartic proteinase family in Candida albicans
Périodique
Microbiology
ISSN
1350-0872 (Print)
Statut éditorial
Publié
Date de publication
10/1998
Volume
144 ( Pt 10)
Pages
2731-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Secreted aspartic proteinases (Saps) contribute to the virulence of Candida albicans in systemic animal models of infection. Seven genes encoding Saps (SAP1-SAP7) have been identified to date but evidence suggested the existence of additional SAP genes. The screening of a C. albicans lambda EMBL3 genomic library for the presence of other SAP genes was undertaken. Two new genes, SAP8 and SAP9, were isolated. The N-terminal amino acid sequence deduced from SAP8 downstream of a Kex2p-like cleavage site corresponds to the N-terminal amino acid sequence of the 41 kDa Sap isolated and characterized previously. SAP8 mRNA was expressed preferentially in yeasts at 25 degrees C after 6 and 9 h growth in BSA-containing medium. SAP9 encodes an aspartic proteinase with a Kex2p-like cleavage site and contains a putative glycophosphatidylinositol-anchor signal at the C-terminus. Although the SAP9 gene product has not yet been isolated from cultures of C. albicans, transcripts of SAP9 were observed preferentially in later growth phases when SAP8 expression had decreased.
Mots-clé
Amino Acid Sequence
Aspartic Endopeptidases/chemistry/*genetics/metabolism
Blotting, Northern
Blotting, Southern
Candida albicans/cytology/*genetics
Cell Division
Cloning, Molecular
Fungal Proteins/chemistry/genetics
*Gene Expression Regulation, Fungal
Molecular Sequence Data
Multigene Family
Restriction Mapping
Saccharomyces cerevisiae/genetics
Sequence Alignment
Sequence Homology, Amino Acid
Temperature
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:46
Dernière modification de la notice
20/08/2019 16:48