Hypervariability of biofilm formation and oxacillin resistance in a Staphylococcus epidermidis strain causing persistent severe infection in an immunocompromised patient.

Détails

ID Serval
serval:BIB_CD579EE7A1B8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Hypervariability of biofilm formation and oxacillin resistance in a Staphylococcus epidermidis strain causing persistent severe infection in an immunocompromised patient.
Périodique
Journal of clinical microbiology
Auteur⸱e⸱s
Weisser M., Schoenfelder S.M., Orasch C., Arber C., Gratwohl A., Frei R., Eckart M., Flückiger U., Ziebuhr W.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
07/2010
Peer-reviewed
Oui
Volume
48
Numéro
7
Pages
2407-2412
Langue
anglais
Notes
Publication types: Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We report on a leukemic patient who suffered from a persistent, generalized, and eventually fatal Staphylococcus epidermidis infection during prolonged aplasia. Over a 6-week period, we isolated a genetically and phenotypically unstable S. epidermidis strain related to an epidemic clone associated with hospital infections worldwide. Strikingly, the strain showed a remarkable degree of variability, with evidence of selection and increasing predominance of biofilm-producing and oxacillin-resistant variants over time. Thus, in the early stages of the infection, the strain was found to generate subpopulations which had spontaneously lost the biofilm-mediating ica locus along with the oxacillin resistance-conferring mecA gene. These deletion mutants were obviously outcompeted by the ica- and mecA-positive wild-type genotype, with the selection and predominance of strongly biofilm-forming and oxacillin-resistant variants in the later stages of the infection. Also, a switch from protein- to polysaccharide intercellular adhesin/poly-N-acetylglucosamine (PIA/PNAG)-mediated-biofilm production was detected among ica-positive variants in the course of the infection. The data highlight the impact of distinct S. epidermidis clonal lineages as serious nosocomial pathogens that, through the generation and selection of highly pathogenic variants, may critically determine disease progression and outcome.
Mots-clé
Anti-Bacterial Agents/pharmacology, Anti-Bacterial Agents/therapeutic use, Bacteremia/microbiology, Biofilms/growth & development, Fatal Outcome, Humans, Immunocompromised Host, Male, Microbial Sensitivity Tests, Oxacillin/pharmacology, Oxacillin/therapeutic use, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, Staphylococcal Infections/diagnosis, Staphylococcal Infections/drug therapy, Staphylococcal Infections/microbiology, Staphylococcus epidermidis/drug effects, Staphylococcus epidermidis/pathogenicity, Stem Cell Transplantation, Young Adult, beta-Lactam Resistance
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/11/2019 10:10
Dernière modification de la notice
02/11/2019 6:26
Données d'usage