Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold

Détails

ID Serval
serval:BIB_CC4FC6004183
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold
Périodique
Cell
Auteur(s)
Mirkovitch  J., Mirault  M. E., Laemmli  U. K.
ISSN
0092-8674 (Print)
Statut éditorial
Publié
Date de publication
11/1984
Volume
39
Numéro
1
Pages
223-32
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells. We find one loop for each of the tandemly repeated histone gene clusters. The attachment site is localized in the A + T rich H1-H3 spacer on a 657 bp fragment. In the cluster of the hsp70 heat-shock genes, in both control and heat-shocked cells, we find two attachment sites in close proximity upstream of regulatory elements. The transcribed sequences are not associated with the nuclear scaffold in control or in heat-shocked cells. A family of attachment sites related by hybridization to those of the hsp70 genes was discovered.
Mots-clé
Animals Cell Line Cell Nucleus/*ultrastructure Chromatin/metabolism/*ultrastructure DNA/*isolation & purification DNA Restriction Enzymes Drosophila melanogaster *Genes Histones/*genetics/isolation & purification
Pubmed
Web of science
Création de la notice
25/01/2008 9:41
Dernière modification de la notice
20/08/2019 16:47
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