Induction of human T cell proliferation by a monoclonal antibody to CD5

Détails

ID Serval
serval:BIB_CBBAF0D209CE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of human T cell proliferation by a monoclonal antibody to CD5
Périodique
Journal of Immunology
Auteur(s)
Spertini  F., Stohl  W., Ramesh  N., Moody  C., Geha  R. S.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
01/1991
Volume
146
Numéro
1
Pages
47-52
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan 1
Résumé
A mouse mAb, TS 43, which recognized the human CD5 molecule, was found to induce the proliferation of human peripheral blood T cells. TS 43 mAb precipitated from 125I-radiolabeled T cells a 67-kDa band, which comigrated with the 67-kDa band precipitated by the anti-CD5 mAb OKT1. Preclearing of cell lysates with OKT1 mAb abolished the capacity of TS 43 mAb to precipitate radiolabeled material from T cell lysates. Furthermore, a mouse T cell hybridoma transfected with human CD5 was stained by TS 43 mAb. T cell proliferation mediated by TS 43 mAb was monocyte dependent, and was accompanied by IL-2R expression and by IL-2 synthesis. T cell activation by TS 43 mAb involved a rise in intracellular calcium level (CA2+)i and was dependent on the expression of the TCR/CD3 complex because no rise in (Ca2+)i was observed in a TCR-beta-deficient Jurkat T cell mutant. This study indicates that CD5 should be added to the list of surface molecules that can signal T cells to proliferate.
Mots-clé
Antibodies, Monoclonal/*immunology Antigens, CD/*immunology Antigens, CD3 Antigens, CD5 Antigens, Differentiation/*immunology Antigens, Differentiation, T-Lymphocyte/physiology Calcium/physiology Humans Interleukin-2/biosynthesis *Lymphocyte Activation Receptors, Antigen, T-Cell/physiology Receptors, Interleukin-2/immunology Signal Transduction T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:19
Dernière modification de la notice
20/08/2019 16:46
Données d'usage