Soy supplementation: Impact on gene expression in different tissues of ovariectomized rats and evaluation of the rat model to predict (post)menopausal health effect.

Détails

Ressource 1Télécharger: 30425930_BIB_CB40B5F86381.pdf (1136.96 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_CB40B5F86381
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Etude de cas (case report): rapporte une observation et la commente brièvement.
Collection
Publications
Institution
Titre
Soy supplementation: Impact on gene expression in different tissues of ovariectomized rats and evaluation of the rat model to predict (post)menopausal health effect.
Périodique
Toxicology reports
Auteur(s)
Islam M.A., Hooiveld GJEJ, van den Berg JHJ, van der Velpen V., Murk A.J., Rietjens IMCM, van Leeuwen FXR
ISSN
2214-7500 (Electronic)
ISSN-L
2214-7500
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
5
Pages
1087-1097
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
This toxicogenomic study was conducted to predict (post)menopausal human health effects of commercial soy supplementation using ovariectomized rats as a model. Different target tissues (i.e. breast, uterus and sternum) and non-target tissues (i.e. peripheral blood mononuclear cells (PBMC), adipose and liver) of ovariectomized F344 rats exposed to a commercially available soy supplement for eight weeks, were investigated. Changes in gene expression in these tissues were analysed using whole-genome microarray analysis. No correlation in changes in gene expression were observed among different tissues, indicating tissue specific effects of soy isoflavone supplementation. Out of 87 well-established estrogen responsive genes (ERGs), only 19 were found to be significantly regulated (p < 0.05) in different tissues, particularly in liver, adipose and uterus tissues. Surprisingly, no ERGs were significantly regulated in estrogen sensitive breast and sternum tissues. The changes in gene expression in PBMC and adipose tissue in rats were compared with those in (post)menopausal female volunteers who received the same supplement in a similar oral dose and exposure duration in human intervention studies. No correlation in changes in gene expression between rats and humans was observed. Although receiving a similar dose, in humans the plasma levels expressed as total free aglycones were several folds higher than in the rat. Therefore, the overall results in young ovariectomized female F344 rats indicated that using rat transcriptomic data does not provide a suitable model for human risk or benefit analysis of soy isoflavone supplementation.
Mots-clé
(Post)menopausal health effect, ERGs, estrogen responsive genes, ERα, estrogen receptor alpha, ERβ, estrogen receptor beta, Gene expression, Ovariectomized rat model, PBMC, peripheral blood mononuclear cells, Soy isoflavone supplementation
Pubmed
Open Access
Oui
Création de la notice
20/11/2018 8:20
Dernière modification de la notice
21/08/2019 6:10
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