Alloimmune Monitoring After Islet Transplantation: A Prospective Multicenter Assessment of 25 Recipients

Détails

ID Serval
serval:BIB_CAE69405260F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Alloimmune Monitoring After Islet Transplantation: A Prospective Multicenter Assessment of 25 Recipients
Périodique
Cell Transplantation
Auteur⸱e⸱s
Delaune V., Toso C., Benhamou P. Y., Wojtusciszyn A., Kessler L., Slits F., Demuylder-Mischler S., Pernin N., Lablanche S., Orci L. A., Oldani G., Morel P., Berney T., Lacotte S.
ISSN
0963-6897
Statut éditorial
Publié
Date de publication
2016
Volume
25
Numéro
12
Pages
2259-2268
Langue
anglais
Notes
Ef2vh
Times Cited:2
Cited References Count:35
Résumé
Islet transplantation is an effective treatment for selected patients with type 1 diabetes. However, an accurate test still lacks for the early detection of graft rejection. Blood samples were prospectively collected in four university centers (Geneva, Grenoble, Montpellier, and Strasbourg). Peripheral blood mononuclear cells were stimulated with donor splenocytes in the presence of interleukin-2. After 24 h of incubation, interferon-gamma (IFN-gamma) ELISpot analysis was performed. After a total of 5 days of incubation, cell proliferation was assessed by fluorescence-activated cell sorting (FACS) analysis for Ki-67. Immunological events were correlated with adverse metabolic events determined by loss of >= 1 point of beta-score and/or an increased insulin intake Twenty-five patients were analyzed; 14 were recipients of islets alone, and 11 combined with kidney. Overall, 76% (19/25) reached insulin independence at one point during a mean follow-up of 30.7 months. IFN-gamma ELISpot showed no detectable correlation with adverse metabolic events [area under the curve (AUC)= 0.57]. Similarly, cell proliferation analysis showed no detectable correlation with adverse metabolic events (CD3(+)/CD4(+)AUC = 0.54; CD3(+)/CD8(+) AUC = 0.55; CD3(-)/CD56(+) AUC= 0.50). CD3(-)/CD56(+) cell proliferation was significantly higher in patients with combined kidney transplantation versus islet alone (6 months, p= 0.010; 12 months, p= 0.016; and 24 months, p= 0.018). Donor antigen-stimulated IFNI, production and cell proliferation do not predict adverse metabolic events after islet transplantation. This suggests that the volume of transplanted islets is too small to produce a detectable systemic immune response and/or that alloimmune rejection is not the sole reason for the loss of islet graft function.
Mots-clé
diabetes, islet transplantation, alloimmune monitoring, elispot, cell proliferation, cell transplantation, organ-transplantation, allograft recipients, peripheral-blood, rejection, ligands, nkg2d, graft, incompatibility, alloreactivity
Web of science
Création de la notice
14/06/2021 8:58
Dernière modification de la notice
18/09/2021 5:38
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