A Metabolomic Signature of Acute Caloric Restriction.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_CAD4DFEFC0AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A Metabolomic Signature of Acute Caloric Restriction.
Périodique
The Journal of clinical endocrinology and metabolism
Auteur(s)
Collet T.H., Sonoyama T., Henning E., Keogh J.M., Ingram B., Kelway S., Guo L., Farooqi I.S.
ISSN
1945-7197 (Electronic)
ISSN-L
0021-972X
Statut éditorial
Publié
Date de publication
01/12/2017
Peer-reviewed
Oui
Volume
102
Numéro
12
Pages
4486-4495
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The experimental paradigm of acute caloric restriction (CR) followed by refeeding (RF) can be used to study the homeostatic mechanisms that regulate energy homeostasis, which are relevant to understanding the adaptive response to weight loss.
Metabolomics, the measurement of hundreds of small molecule metabolites, their precursors, derivatives, and degradation products, has emerged as a useful tool for the study of physiology and disease and was used here to study the metabolic response to acute CR.
We used four ultra high-performance liquid chromatography-tandem mass spectrometry methods to characterize changes in carbohydrates, lipids, amino acids, and steroids in eight normal weight men at baseline, after 48 hours of CR (10% of energy requirements) and after 48 hours of ad libitum RF in a tightly controlled environment.
We identified a distinct metabolomic signature associated with acute CR characterized by the expected switch from carbohydrate to fat utilization with increased lipolysis and β-fatty acid oxidation. We found an increase in ω-fatty acid oxidation and levels of endocannabinoids, which are known to promote food intake. These changes were reversed with RF. Several plasmalogen phosphatidylethanolamines (endogenous antioxidants) significantly decreased with CR (all P ≤ 0.0007). Additionally, acute CR was associated with an increase in the branched chain amino acids (all P ≤ 1.4 × 10-7) and dehydroepiandrosterone sulfate (P = 0.0006).
We identified a distinct metabolomic signature associated with acute CR. Further studies are needed to characterize the mechanisms that mediate these changes and their potential contribution to the adaptive response to dietary restriction.

Mots-clé
Adult, Amino Acids/metabolism, Antioxidants/metabolism, Caloric Restriction, Carbohydrate Metabolism/physiology, Chromatography, High Pressure Liquid, Dehydroepiandrosterone Sulfate/metabolism, Endocannabinoids/metabolism, Energy Metabolism/physiology, Fatty Acids/metabolism, Homeostasis, Humans, Lipid Metabolism/physiology, Lipolysis, Male, Metabolomics/methods, Oxidation-Reduction, Steroids/blood, Tandem Mass Spectrometry, Weight Loss/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/10/2017 14:28
Dernière modification de la notice
20/08/2019 15:45
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