Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.

Détails

ID Serval
serval:BIB_CA638E2C15CF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.
Périodique
Cell
Auteur⸱e⸱s
Gruber S., Errington J.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
137
Numéro
4
Pages
685-696
Langue
anglais
Résumé
Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and Spo0J, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which Spo0J binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large region around the origin of replication depends on the presence of Spo0J. We show that Spo0J performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment. Our results demonstrate a direct functional interaction between two widely conserved systems involved in chromosome replication and segregation.
Mots-clé
Adenosine Triphosphatases/metabolism, Bacillus subtilis/chemistry, Bacillus subtilis/genetics, Bacterial Proteins/metabolism, Cell Cycle Proteins/metabolism, Chromosomes, Bacterial/metabolism, DNA-Binding Proteins/metabolism, Gene Deletion, Multiprotein Complexes/metabolism, Operon, Replication Origin
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/08/2016 9:52
Dernière modification de la notice
20/08/2019 15:45
Données d'usage