Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.

Détails

ID Serval
serval:BIB_C98D8C03C4DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.
Périodique
Journal of Molecular Biology
Auteur⸱e⸱s
Herr W., Noller H.F.
ISSN
0022-2836 (Print)
ISSN-L
0022-2836
Statut éditorial
Publié
Date de publication
1979
Volume
130
Numéro
4
Pages
421-432
Langue
anglais
Résumé
The effect of 30S subunit attachment on the accessibility of specific sites in 5 S and 23 S RNA in 50 S ribosomal subunits was studied by means of the guanine-specific reagent kethoxal. Oligonucleotides surrounding the sites of kethoxal substitution were resolved and quantitated by diagonal electrophoresis. In contrast to the extensive protection of sites in 16 S RNA in 70 S ribosomes (Chapman & Noller, 1977), only two strongly (approx. 90%) protected sites were detected in 23 S RNA. The nucleotide sequences at these sites are
in which the indicated kethoxal-reactive guanines (with K above them) are strongly protected by association of 30 S and 50 S subunits. The latter sequence has the potential to base-pair with nucleotides 816 to 821 of the 16 S RNA, a site which has been shown to be protected from kethoxal by 50 S subunits and essential for subunit association. Six additional sites in 23 S RNA are partially (30 to 50%) protected by 30 S subunits. One of these sequences, is complementary to nucleotides 787 to 792 of 16 S RNA. a site which is also 50 S-protected and essential for association. Of the two kethoxal-reactive 5 S RNA sites in 50 S subunits, G13 is partially protected in 70 S ribosomes. while G41 remains unaffected by subunit association.
The relatively small number of kethoxal-reactive sites in 23 S RNA that is strongly protected in 70 S ribosomes suggests that subunit association may involve contacts between single-stranded sites in 16 S RNA and 50 S subunit proteins or non-Watson-Crick interactions with 23 S RNA. in addition to the two suggested base-paired contacts.
Mots-clé
Aldehydes/pharmacology, Base Sequence, Binding Sites/drug effects, Butanones/pharmacology, Electrophoresis, Escherichia coli/drug effects, Nucleic Acid Hybridization, RNA, Bacterial, RNA, Ribosomal, Ribonucleotides/analysis, Ribosomes/drug effects
Pubmed
Web of science
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 15:44
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