Cortical Bilateral Adaptations in Rats Submitted to Focal Cerebral Ischemia: Emphasis on Glial Metabolism.

Détails

ID Serval
serval:BIB_C952FB2A4F43
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cortical Bilateral Adaptations in Rats Submitted to Focal Cerebral Ischemia: Emphasis on Glial Metabolism.
Périodique
Molecular neurobiology
Auteur(s)
Nonose Y., Gewehr P.E., Almeida R.F., da Silva J.S., Bellaver B., Martins LAM, Zimmer E.R., Greggio S., Venturin G.T., Da Costa J.C., Quincozes-Santos A., Pellerin L., de Souza D.O., de Assis A.M.
ISSN
1559-1182 (Electronic)
ISSN-L
0893-7648
Statut éditorial
Publié
Date de publication
03/2018
Peer-reviewed
Oui
Volume
55
Numéro
3
Pages
2025-2041
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
This study was performed to evaluate the bilateral effects of focal permanent ischemia (FPI) on glial metabolism in the cerebral cortex. Two and 9 days after FPI induction, we analyze [ <sup>18</sup> F]FDG metabolism by micro-PET, astrocyte morphology and reactivity by immunohistochemistry, cytokines and trophic factors by ELISA, glutamate transporters by RT-PCR, monocarboxylate transporters (MCTs) by western blot, and substrate uptake and oxidation by ex vivo slices model. The FPI was induced surgically by thermocoagulation of the blood in the pial vessels of the motor and sensorimotor cortices in adult (90 days old) male Wistar rats. Neurochemical analyses were performed separately on both ipsilateral and contralateral cortical hemispheres. In both cortical hemispheres, we observed an increase in tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and glutamate transporter 1 (GLT-1) mRNA levels; lactate oxidation; and glutamate uptake and a decrease in brain-derived neurotrophic factor (BDNF) after 2 days of FPI. Nine days after FPI, we observed an increase in TNF-α levels and a decrease in BDNF, GLT-1, and glutamate aspartate transporter (GLAST) mRNA levels in both hemispheres. Additionally, most of the unilateral alterations were found only in the ipsilateral hemisphere and persisted until 9 days post-FPI. They include diminished in vivo glucose uptake and GLAST expression, followed by increased glial fibrillary acidic protein (GFAP) gray values, astrocyte reactivity, and glutamate oxidation. Astrocytes presented signs of long-lasting reactivity, showing a radial morphology. In the intact hemisphere, there was a decrease in MCT2 levels, which did not persist. Our study shows the bilateralism of glial modifications following FPI, highlighting the role of energy metabolism adaptations on brain recovery post-ischemia.
Mots-clé
Adaptation, Physiological/physiology, Animals, Brain Ischemia/metabolism, Brain Ischemia/pathology, Cerebral Cortex/metabolism, Cerebral Cortex/pathology, Excitatory Amino Acid Transporter 1/metabolism, Excitatory Amino Acid Transporter 2/metabolism, Male, Neuroglia/metabolism, Neuroglia/pathology, Rats, Rats, Wistar, Astrocytes, Contralateral hemisphere, Energy metabolism, Glial reactivity, Stroke
Pubmed
Web of science
Création de la notice
14/03/2017 10:12
Dernière modification de la notice
22/08/2019 8:26
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