Comparison of a multiplexed bead-based assay with an immunofluorescence and an enzyme-immuno assay for the assessment of Epstein-Barr virus serologic status.

Détails

ID Serval
serval:BIB_C90CDFF4BBE8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of a multiplexed bead-based assay with an immunofluorescence and an enzyme-immuno assay for the assessment of Epstein-Barr virus serologic status.
Périodique
Clinical Microbiology and Infection
Auteur(s)
Devanthéry O., Meylan P.
ISSN
1469-0691[electronic], 1198-743X[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
16
Numéro
12
Pages
1776-1782
Langue
anglais
Résumé
We have compared a multiplexed bead-based assay (BBA) with an enzyme immunoassay (EIA) and immunofluorescence assay (IFA) for the assessment of the Epstein-Barr virus (EBV) serostatus. Three hundred and ninety-three sera, classified according to IFA results as seronegative (n=100), acute infection (n=100), past infection (n=100) and indeterminate (n=93), were tested by BBA and EIA. Overall, the three methods gave similar results with a relatively high (75.2%) concordance with the consensus interpretation of the serostatus. The most significant discordances were: (i) 58 samples had uninterpretable results for BBA, in majority due to the detection of non-antigen specific antibody binding by control beads. (ii) almost half the samples positive for anti-Epstein-Barr nuclear antigen (EBNA) IgG by BBA or EIA were negative by IFA. Among the latter, only a minority had a history of immunocompromise or treatment, or detectable anti-early antigen antibody. This discrepancy probably reflects a poor sensitivity of IFA for anti-EBNA IgG detection. EIA and BBA had a similar performance and had substantial practical advantages over IFA with respect to testing for EBV serostatus.
Mots-clé
Epstein-Barr Virus, ELISA, Immunofluorescence, Luminex, Serology, Antibody, Titers
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/12/2010 11:24
Dernière modification de la notice
20/08/2019 16:44
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