High-performance liquid chromatography-ionspray mass spectrometry for the specific determination of digoxin and some related cardiac glycosides in human plasma.

Détails

ID Serval
serval:BIB_C900A770E0E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High-performance liquid chromatography-ionspray mass spectrometry for the specific determination of digoxin and some related cardiac glycosides in human plasma.
Périodique
Journal of Chromatography. B, Biomedical Sciences and Applications
Auteur(s)
Tracqui A., Kintz P., Ludes B., Mangin P.
ISSN
1387-2273[print], 1387-2273[linking]
Statut éditorial
Publié
Date de publication
1997
Volume
692
Numéro
1
Pages
101-109
Langue
anglais
Résumé
An original method based upon high-performance liquid chromatography coupled to ionspray mass spectrometry (HPLC-ISP-MS) has been developed for the identification and quantification in plasma of several cardiac glycosides, namely digoxin, digitoxin, lanatoside C and acetyldigitoxin. After single-step liquid-liquid extraction by chloroform-2-propanol (95:5, v/v) at pH 9.5 using oleandrin as an internal standard, solutes are separated on a 4 microm NovaPak C18 (Waters) column (150x2.0 mm, I.D.), using a gradient of acetonitrile-2 mM NH4COOH, pH 3 buffer (flow-rate 200 microl/min, post-column split 1:3). Detection is done by a Perkin-Elmer Sciex API-100 mass analyzer equipped with an ISP interface. In most instances the major ion observed is not [M+H]+ as expected, but [M+NH4]+. The mean retention times (min) are: lanatoside C, 5.74; digoxin, 6.00; digitoxin, 8.08, oleandrin, 8.30, acetyldigitoxin, 8.66 and 9.01 (isomers alpha and beta, respectively). The lower limits of detection in single ion monitoring mode range from 0.15 ng/ml (alpha- and beta-acetyldigitoxin) to 0.60 ng/ml (lanatoside C), making the method less sensitive than radioimmunoassay, whereas it is much more specific.
Mots-clé
Acetyldigitoxins/blood, Cardiac Glycosides/blood, Cardiotonic Agents/blood, Chromatography, High Pressure Liquid, Digitoxin/blood, Digoxin/blood, Gas Chromatography-Mass Spectrometry, Humans, Lanatosides/blood, Ouabain/blood, Sensitivity and Specificity
Pubmed
Création de la notice
19/10/2010 17:13
Dernière modification de la notice
20/08/2019 16:44
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