Heterogeneity of Radial Glia-Like Cells in the Adult Hippocampus.

Détails

Ressource 1Télécharger: 5_26729510_Postprint.pdf (5244.42 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_C8FE2775CCE3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Heterogeneity of Radial Glia-Like Cells in the Adult Hippocampus.
Périodique
Stem cells
Auteur(s)
Gebara E., Bonaguidi M.A., Beckervordersandforth R., Sultan S., Udry F., Gijs P.J., Lie D.C., Ming G.L., Song H., Toni N.
ISSN
1549-4918 (Electronic)
ISSN-L
1066-5099
Statut éditorial
Publié
Date de publication
04/2016
Peer-reviewed
Oui
Volume
34
Numéro
4
Pages
997-1010
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Adult neurogenesis is tightly regulated by the neurogenic niche. Cellular contacts between niche cells and neural stem cells are hypothesized to regulate stem cell proliferation or lineage choice. However, the structure of adult neural stem cells and the contact they form with niche cells are poorly described. Here, we characterized the morphology of radial glia-like (RGL) cells, their molecular identity, proliferative activity, and fate determination in the adult mouse hippocampus. We found the coexistence of two morphotypes of cells with prototypical morphological characteristics of RGL stem cells: Type α cells, which represented 76% of all RGL cells, displayed a long primary process modestly branching into the molecular layer and type β cells, which represented 24% of all RGL cells, with a shorter radial process highly branching into the outer granule cell layer-inner molecular layer border. Stem cell markers were expressed in type α cells and coexpressed with astrocytic markers in type β cells. Consistently, in vivo lineage tracing indicated that type α cells can give rise to neurons, astrocytes, and type β cells, whereas type β cells do not proliferate. Our results reveal that the adult subgranular zone of the dentate gyrus harbors two functionally different RGL cells, which can be distinguished by simple morphological criteria, supporting a morphofunctional role of their thin cellular processes. Type β cells may represent an intermediate state in the transformation of type α, RGL stem cells, into astrocytes.

Mots-clé
Animals, Biomarkers/metabolism, Cell Lineage/genetics, Cell Proliferation, Ependymoglial Cells/cytology, Ependymoglial Cells/metabolism, Ependymoglial Cells/transplantation, Hippocampus/cytology, Hippocampus/pathology, Humans, Mice, Neural Stem Cells/cytology, Neural Stem Cells/metabolism, Neural Stem Cells/transplantation, Neurogenesis, Adult stem cells, Nervous system, Neural stem cell, Somatic stem cells, Stem cell-microenvironment interactions
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2016 8:26
Dernière modification de la notice
20/08/2019 16:44
Données d'usage