Comparison of two assays for the determination of specific IgE in serum of atopic and nonatopic subjects: the Allergenetics FAST and the Phadezym RAST

Détails

ID Serval
serval:BIB_C8C4FF489CEF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of two assays for the determination of specific IgE in serum of atopic and nonatopic subjects: the Allergenetics FAST and the Phadezym RAST
Périodique
Allergy
Auteur(s)
Pecoud  A., Peitrequin  R., Fasel  J., Frei  P. C.
ISSN
0105-4538 (Print)
Statut éditorial
Publié
Date de publication
05/1986
Volume
41
Numéro
4
Pages
243-9
Notes
Comparative Study
Journal Article --- Old month value: May
Résumé
The FAST assay (Allergenetics) for the determination of specific IgE has recently been introduced. The results of this test, graded in classes 0 (negative), 1 (equivocal) and 2 to 6 (positive) were compared with those of another commercially available test (Phadezym RAST, Pharmacia), graded from class 0 (negative) and classes 1 to 4 (positive). In 52 adults suffering from rhinitis/asthma, a total of 114 positive skin prick tests (SPT) were obtained for common respiratory allergens. In 67% of these tests the Phadezym RAST was positive and in 63% the FAST was positive (classes 2 to 6). In these patients there were 151 negative SPT: 6% corresponded to a positive Phadezym RAST (all class 1) and 34% to a positive FAST (classes 2 to 6). The serum of nine nonatopic volunteers who had negative SPT for 12 common allergens were tested. In none did the Phadezym RAST give any positive results; the FAST was positive in all nine sera, detecting between five and 11 allergens. When both assays were performed on 14 unselected cord blood samples, the Phadezym RAST was positive in three samples (with class 1 results to a maximum of two allergens), and the FAST was positive in 12 samples, detecting between one and seven allergens. Thus, in our hands, the FAST gave an abnormally high number of positive results in patients with negative SPT, in nonatopic volunteers, and in cord blood.
Mots-clé
Adolescent Adult Allergens/immunology Antibody Specificity Evaluation Studies False Positive Reactions Female Fetal Blood/immunology Humans Hypersensitivity, Immediate/*immunology Immunoglobulin E/*analysis Infant, Newborn Male Middle Aged *Radioallergosorbent Test *Radioimmunoassay Skin Tests
Pubmed
Web of science
Création de la notice
28/01/2008 12:58
Dernière modification de la notice
20/08/2019 16:43
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