Opposite regulation of calbindin and calretinin expression by brain-derived neurotrophic factor in cortical neurons.

Détails

ID Serval
serval:BIB_C8B6F9D58957
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Opposite regulation of calbindin and calretinin expression by brain-derived neurotrophic factor in cortical neurons.
Périodique
Journal of neurochemistry
Auteur⸱e⸱s
Fiumelli H., Kiraly M., Ambrus A., Magistretti P.J., Martin J.L.
ISSN
0022-3042
Statut éditorial
Publié
Date de publication
05/2000
Peer-reviewed
Oui
Volume
74
Numéro
5
Pages
1870-7
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Regulation of calbindin and calretinin expression by brain-derived neurotrophic factor (BDNF) was examined in primary cultures of cortical neurons using immunocytochemistry and northern blot analysis. Here we report that regulation of calretinin expression by BDNF is in marked contrast to that of calbindin. Indeed, chronic exposure of cultured cortical neurons for 5 days to increasing concentrations of BDNF (0.1-10 ng/ml) resulted in a concentration-dependent decrease in the number of calretinin-positive neurons and a concentration-dependent increase in the number of calbindin-immunoreactive neurons. Consistent with the immunocytochemical analysis, BDNF reduced calretinin mRNA levels and up-regulated calbindin mRNA expression, providing evidence that modifications in gene expression accounted for the changes in the number of calretinin- and calbindin-containing neurons. Among other members of the neurotrophin family, neurotrophin-4 (NT-4), which also acts by activating tyrosine kinase TrkB receptors, exerted effects comparable to those of BDNF, whereas nerve growth factor (NGF) was ineffective. As for BDNF and NT-4, incubation of cortical neurons with neurotrophin-3 (NT-3) also led to a decrease in calretinin expression. However, in contrast to BDNF and NT-4, NT-3 did not affect calbindin expression. Double-labeling experiments evidenced that calretinin- and calbindin-containing neurons belong to distinct neuronal subpopulations, suggesting that BDNF and NT-4 exert opposite effects according to the neurochemical phenotype of the target cell.
Mots-clé
Animals, Brain-Derived Neurotrophic Factor, Calcium-Binding Protein, Vitamin D-Dependent, Cells, Cultured, Cerebral Cortex, Immunohistochemistry, Mice, Nerve Growth Factors, Neurons, RNA, Messenger
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2008 19:22
Dernière modification de la notice
20/08/2019 16:43
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