Molecular dissection of the events leading to inactivation of the FMR1 gene.

Détails

ID Serval
serval:BIB_C7BF80614DB4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular dissection of the events leading to inactivation of the FMR1 gene.
Périodique
Human molecular genetics
Auteur⸱e⸱s
Pietrobono R., Tabolacci E., Zalfa F., Zito I., Terracciano A., Moscato U., Bagni C., Oostra B., Chiurazzi P., Neri G.
ISSN
0964-6906 (Print)
ISSN-L
0964-6906
Statut éditorial
Publié
Date de publication
15/01/2005
Volume
14
Numéro
2
Pages
267-277
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The analysis of a lymphoblastoid cell line (5106), derived from a rare individual of normal intelligence with an unmethylated full mutation of the FMR1 gene, allowed us to reconstruct the chain of molecular events leading to the FMR1 inactivation and to fragile X syndrome. We found that lack of DNA methylation of the entire promoter region, including the expanded CGG repeat, correlates with methylation of lysine 4 residue on the N-tail of histone H3 (H3-K4), as in normal controls. Normal levels of FMR1 mRNA were detected by real-time fluorescent RT-PCR (0.8-1.4 times compared with a control sample), but mRNA translation was less efficient (-40%), as judged by polysome profiling, resulting in reduced levels of FMRP protein (approximately 30% of a normal control). These results underline once more that CGG repeat amplification per se does not prevent FMR1 transcription and FMRP production in the absence of DNA methylation. Surprisingly, we found by chromatin immunoprecipitation that cell line 5106 has deacetylated histones H3 and H4 as well as methylated lysine 9 on histone H3 (H3-K9), like fragile X cell lines, in both the promoter and exon 1. This indicates that these two epigenetic marks (i.e. histone deacetylation and H3-K9 methylation) can be established in the absence of DNA methylation and do not interfere with active gene transcription, contrary to expectation. Our results also suggest that the molecular pathways regulating DNA and H3-K4 methylation are independent from those regulating histone acetylation and H3-K9 methylation.

Mots-clé
Epigenesis, Genetic, Female, Fragile X Mental Retardation Protein, Gene Silencing, Humans, Male, Mutation, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism
Pubmed
Open Access
Oui
Création de la notice
06/03/2017 17:23
Dernière modification de la notice
20/08/2019 15:43
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