Precision and scaling in morphogen gradient read-out.
Détails
Télécharger: BIB_C6BCC0E0674B.P001.pdf (439.60 [Ko])
Etat: Public
Version: de l'auteur⸱e
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_C6BCC0E0674B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Precision and scaling in morphogen gradient read-out.
Périodique
Molecular Systems Biology
ISSN
1744-4292[electronic], 1744-4292[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
6
Pages
351
Langue
anglais
Résumé
Morphogen gradients infer cell fate as a function of cellular position. Experiments in Drosophila embryos have shown that the Bicoid (Bcd) gradient is precise and exhibits some degree of scaling. We present experimental results on the precision of Bcd target genes for embryos with a single, double or quadruple dose of bicoid demonstrating that precision is highest at mid-embryo and position dependent, rather than gene dependent. This confirms that the major contribution to precision is achieved already at the Bcd gradient formation. Modeling this dynamic process, we investigate precision for inter-embryo fluctuations in different parameters affecting gradient formation. Within our modeling framework, the observed precision can only be achieved by a transient Bcd profile. Studying different extensions of our modeling framework reveals that scaling is generally position dependent and decreases toward the posterior pole. Our measurements confirm this trend, indicating almost perfect scaling except for anterior most expression domains, which overcompensate fluctuations in embryo length.
Mots-clé
Bicoid, Drosophila, Morphogen Gradients, Precision, Scaling, Early Drosophila Embryo, Positional Information, Bicoid Gradient, Establishment, Protein, Melanogaster, Proportions, Diffusion, Dynamics, Pattern
Pubmed
Web of science
Open Access
Oui
Création de la notice
23/04/2010 9:06
Dernière modification de la notice
20/08/2019 15:42