Expression of glutamate transporters in the medial and lateral vestibular nuclei during rat postnatal development.

Détails

ID Serval
serval:BIB_C6B387D93FE6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of glutamate transporters in the medial and lateral vestibular nuclei during rat postnatal development.
Périodique
Developmental neuroscience
Auteur⸱e⸱s
Devau G., Plachez C., Puyal J., Pierrot N., Ventéo S., Raymond J.
ISSN
0378-5866
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
25
Numéro
5
Pages
332-423
Langue
anglais
Résumé
The postnatal developmental expression and the distribution of the glutamate transporters (GLAST, GLT-1 and EAAC1) were analyzed in rat vestibular nuclei (VN), at birth and during the following 4 weeks. Analyses were performed using reverse transcriptase-polymerase chain reaction and immunoblotting of GLAST, GLT-1 and EAAC1 mRNA and protein during the postnatal development of the VN neurons and their afferent connections. We also studied the distribution of each glutamate transporter in the medial and lateral VN by use of immunocytochemistry and confocal microscopy. GLAST, GLT-1 and EAAC1 mRNA and protein were present in the VN at each developmental stage. GLAST was highly expressed mainly in glia from birth to the adult stage, its distribution pattern was heterogeneous depending on the region of the medial and lateral VN. GLT-1 expression increased dramatically during the second and third postnatal weeks. At least during the first postnatal week, GLT-1 was expressed in the soma of neurons. EAAC1 was detected in neurons and decreased from the third week. These temporal and regional patterns of GLAST, GLT-1 and EAAC1 suggest that they play different roles in the maturation of glutamatergic synaptic transmission in the medial and lateral VN during postnatal development.
Mots-clé
Amino Acid Transport System X-AG/biosynthesis, Animals, Biological Transport/physiology, Blotting, Western, Excitatory Amino Acid Transporter 1, Excitatory Amino Acid Transporter 2/biosynthesis, Excitatory Amino Acid Transporter 3, Glutamate Plasma Membrane Transport Proteins, Immunohistochemistry, Microscopy, Confocal, Neuroglia/metabolism, Neurons/metabolism, RNA, Messenger, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Symporters/biosynthesis, Vestibular Nuclei/growth & development, Vestibular Nuclei/metabolism
Pubmed
Web of science
Création de la notice
17/09/2009 10:45
Dernière modification de la notice
20/08/2019 15:42
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