The reorganization of microtubules and microfilaments in differentiating keratinocytes
Détails
ID Serval
serval:BIB_C3BCAA472638
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The reorganization of microtubules and microfilaments in differentiating keratinocytes
Périodique
Differentiation
ISSN
0301-4681 (Print)
Statut éditorial
Publié
Date de publication
1987
Volume
36
Numéro
3
Pages
228-33
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S.
Research Support, U.S. Gov't, P.H.S.
Résumé
Using immunofluorescence techniques, we have examined the microtubules and microfilaments in colonies of terminally differentiating human keratinocytes in tissue culture. The undifferentiated keratinocytes contained numerous microtubules, which radiated from a centrosomal organization center (MTOC). Differentiating keratinocytes, which leave the basal layer and begin to synthesize involucrin, displayed an altered cytoskeleton. Thick mats and coils of microtubules formed throughout the cytoplasm of the differentiated squames, and microfilaments were no longer visible after staining with phalloidin. Instead, only scattered stipples of phalloidin-stained material were observed. The results suggest that the terminal differentiation of epidermal cells involves a reorganization not only of the keratin filaments but of the entire cytoskeleton.
Mots-clé
Cell Differentiation
Cells, Cultured
Cytoskeleton/*physiology
Epidermis/*cytology/metabolism
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Keratins/metabolism/*physiology
Microfilaments/metabolism/*physiology/ultrastructure
Microscopy, Electron
Microtubules/metabolism/*physiology/ultrastructure
Pubmed
Web of science
Création de la notice
28/01/2008 8:41
Dernière modification de la notice
20/08/2019 15:39