Building synthetic gene circuits from combinatorial libraries: screening and selection strategies.

Détails

Ressource 1Télécharger: Pub_MolBioSyst2013.pdf (1795.76 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_C2C3379A8E35
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Building synthetic gene circuits from combinatorial libraries: screening and selection strategies.
Périodique
Molecular bioSystems
Auteur⸱e⸱s
Schaerli Y., Isalan M.
ISSN
1742-2051 (Electronic)
ISSN-L
1742-2051
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
9
Numéro
7
Pages
1559-1567
Langue
anglais
Résumé
The promise of wide-ranging biotechnology applications inspires synthetic biologists to design novel genetic circuits. However, building such circuits rationally is still not straightforward and often involves painstaking trial-and-error. Mimicking the process of natural selection can help us to bridge the gap between our incomplete understanding of nature's design rules and our desire to build functional networks. By adopting the powerful method of directed evolution, which is usually applied to protein engineering, functional networks can be obtained through screening or selecting from randomised combinatorial libraries. This review first highlights the practical options to introduce combinatorial diversity into gene circuits and then examines strategies for identifying the potentially rare library members with desired functions, either by screening or selection.

Mots-clé
Gene Library, Gene Regulatory Networks, Genes, Synthetic, Genetic Engineering, High-Throughput Screening Assays, Protein Engineering, Synthetic Biology
Pubmed
Web of science
Création de la notice
02/02/2017 8:08
Dernière modification de la notice
20/08/2019 15:37
Données d'usage