Ionizing radiation enhances immunogenicity of cells expressing a tumor-specific T-cell epitope.

Détails

ID Serval
serval:BIB_C1EF23BC7614
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ionizing radiation enhances immunogenicity of cells expressing a tumor-specific T-cell epitope.
Périodique
International journal of radiation oncology, biology, physics
Auteur⸱e⸱s
Ciernik I.F., Romero P., Berzofsky J.A., Carbone D.P.
ISSN
0360-3016
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
45
Numéro
3
Pages
735-41
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. - Publication Status: ppublish
Résumé
BACKGROUND: p53 point mutations represent potential tumor-specific cytolytic T lymphocyte (CTL) epitopes. Whether ionizing radiation (IR) alters the immunological properties of cells expressing mutant p53 in respect of the CTL epitope generated by a defined point mutation has not been evaluated. METHODS: Mutant p53-expressing syngeneic, nontumor forming BALB/c 3T3 fibroblasts, tumor forming ras-transfected BALB/c 3T3 sarcomas, and DBA/2-derived P815 mastocytoma cells, which differ at the level of minor histocompatibility antigens, were used as cellular vaccines. Cells were either injected with or without prior IR into naive BALB/c mice. Cellular cytotoxicity was assessed after secondary restimulation of effector spleen cells in vitro. RESULTS: Injection of P815 mastocytoma cells expressing the mutant p53 induced mutation-specific CTL in BALB/c mice irrespective of prior irradiation. However, syngeneic fibroblasts or fibrosarcomas endogenously expressing mutant p53 were able to induce significant mutation-specific CTL only when irradiated prior to injection into BALB/c mice. IR of fibroblasts did not detectably alter the expression of cell surface molecules involved in immune response induction, nor did it alter the short-term in vitro viability of the fibroblasts. Interestingly, radioactively-labeled fibroblasts injected into mice after irradiation showed altered organ distribution, suggesting that the in vivo fate of these cells may play a crucial role in their immunogenicity. CONCLUSIONS: These findings indicate that IR can alter the immunogenicity of syngeneic normal as well as tumor forming fibroblasts in vivo, and support the view that ionizing radiation enhances immunogenicity of cellular tumor vaccines.
Mots-clé
3T3 Cells, Animals, Cancer Vaccines, Epitopes, T-Lymphocyte, Female, Genes, p53, Humans, Immunity, Cellular, Mast-Cell Sarcoma, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Point Mutation, Radiobiology, Sarcoma, Experimental, T-Lymphocytes, Cytotoxic, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
28/01/2008 12:27
Dernière modification de la notice
20/08/2019 16:36
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