Testing human hair for Cannabis. III. rapid screening procedure for the simultaneous identification of delta 9-tetrahydrocannabinol, cannabinol, and cannabidiol.

Détails

ID Serval
serval:BIB_C1718CB30FAC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Testing human hair for Cannabis. III. rapid screening procedure for the simultaneous identification of delta 9-tetrahydrocannabinol, cannabinol, and cannabidiol.
Périodique
Journal of Analytical Toxicology
Auteur⸱e⸱s
Cirimele V., Sachs H., Kintz P., Mangin P.
ISSN
0146-4760[print], 0146-4760[linking]
Statut éditorial
Publié
Date de publication
1996
Volume
20
Numéro
1
Pages
13-16
Langue
anglais
Résumé
delta 9-Tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN) are three constituents of the 16 that can be currently isolated from some Cannabis spp plants. Their identification in decontaminated hair can indicate exposure to cannabis. In this study, we propose a rapid, simple, and direct (without derivatization) screening procedure for the simultaneous identification and quantitation of CBD, CBN, and THC in hair of chronic cannabis abusers. Hair samples were washed with methylene chloride, hydrolyzed with sodium hydroxide, extracted with n-hexane-ethyl acetate (9:1, v/v), evaporated to dryness, and injected directly on a gas chromatographic-mass spectrometric system operating in electron-impact mode. THC-d3 was used as the internal standard. Thirty hair samples were tested. CBD was detected 23 times, CBN was detected 22 times, and THC was detected five times. Concentrations ranged from 0.03 to 3.00 ng/mg (mean, 0.44 ng/mg), from 0.01 to 1.07 ng/mg (mean, 0.13 ng/mg), and from 0.1 to 0.29 ng/mg hair (mean, 0.15 ng/mg) for CBD, CBN, and THC, respectively. These results show that this new screening procedure is suitable for the detection of CBD and CBN in the hair of cannabis abusers.
Mots-clé
Adolescent, Adult, Cannabidiol/analysis, Cannabidiol/chemistry, Cannabinol/analysis, Cannabinol/chemistry, Cannabis/chemistry, Drug Evaluation, Preclinical/methods, Hair/chemistry, Humans, Mass Spectrometry/methods, Sensitivity and Specificity, Substance-Related Disorders, Tetrahydrocannabinol/analysis, Tetrahydrocannabinol/chemistry
Pubmed
Création de la notice
19/10/2010 16:47
Dernière modification de la notice
20/08/2019 16:36
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