Receptor-mediated transcellular transport of immunoglobulin: synthesis of secretory component as multiple and larger transmembrane forms

Détails

ID Serval
serval:BIB_C1664FF9190D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Receptor-mediated transcellular transport of immunoglobulin: synthesis of secretory component as multiple and larger transmembrane forms
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Mostov  K. E., Kraehenbuhl  J. P., Blobel  G.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
12/1980
Volume
77
Numéro
12
Pages
7257-61
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec
Résumé
We have characterized the early biosynthetic forms of secretory component (SC). SC is synthesized in various glandular epithelial cells and functions in transepithelial transport of certain polymeric immunoglobulins. In rabbit, mature SC is known to be heterogeneous, consisting of two or three immunologically related glycoproteins. Using translation of mRNA from rabbit mammary gland or liver in a wheat germ cell-free system supplemented with dog pancreas microsomal vesicles, we discovered that the translation products of SC mRNA include at least four distinct polypeptides. Moreover, we found that all four polypeptides are synthesized not as soluble secretory forms but as considerably larger transmembrane forms that are core-glycosylated and asymmetrically integrated into the dog pancreas microsomal vesicles in a translation-coupled manner. We therefore conclude that the mature secreted forms of SC are endoproteolytic fragments derived from and comprising the ectoplasmic portion of the transmembrane precursor forms. The detection of transmembrane precursor forms for mature SC provides a protein structural basis for their function as receptors mediating transepithelial transport of immuno-globulin molecules.
Mots-clé
Cell-Free System Glycoproteins/metabolism Immunoglobulin A/metabolism Immunoglobulin Fragments/*biosynthesis Intracellular Membranes/metabolism Membrane Proteins/metabolism Microsomes/metabolism Molecular Weight Protein Biosynthesis Protein Precursors/*biosynthesis Secretory Component/*biosynthesis
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 15:36
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