A bioluminescent-based probe for in vivo non-invasive monitoring of nicotinamide riboside uptake reveals a link between metastasis and NAD+ metabolism.

Détails

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Accès restreint UNIL
Etat: Public
Version: de l'auteur⸱e
Licence: Non spécifiée
ID Serval
serval:BIB_C15EADDDA9C7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A bioluminescent-based probe for in vivo non-invasive monitoring of nicotinamide riboside uptake reveals a link between metastasis and NAD+ metabolism.
Périodique
Biosensors & bioelectronics
Auteur⸱e⸱s
Maric T., Bazhin A., Khodakivskyi P., Mikhaylov G., Solodnikova E., Yevtodiyenko A., Giordano Attianese GMP, Coukos G., Irving M., Joffraud M., Cantó C., Goun E.
ISSN
1873-4235 (Electronic)
ISSN-L
0956-5663
Statut éditorial
Publié
Date de publication
15/01/2023
Peer-reviewed
Oui
Volume
220
Pages
114826
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Nicotinamide riboside (NR) is a form of vitamin B <sub>3</sub> and is one of the most studied compounds for the restoration of cellular NAD <sup>+</sup> levels demonstrating clinical potential in many metabolic and age-related disorders. Despite its wide commercial availability as a powerful nutraceutical, our understanding of NR uptake by different cells and tissues is greatly limited by the lack of noninvasive in vivo imaging tools limiting its clinical translation. Here, we report the development and validation of a bioluminescent NR uptake probe (BiNR) for non-invasive longitudinal imaging of NR uptake both in vitro and in vivo. In addition, we optimized an assay that allows monitoring of NR flux without the need to transfect cells with the luciferase gene, enabling the use of the BiNR probe in clinical samples, as demonstrated with human T cells. Lastly, we used BiNR to investigate the role of NR uptake in cancer prevalence and metastases formation in triple negative breast cancer (TNBC) animal model. Our results demonstrate that NR supplementation results in a significant increase in cancer prevalence and metastases of TNBC to the brain. These results outline the important role of powerful nutraceuticals like NR in cancer metabolism and the need to personalize their use in certain patient populations.
Mots-clé
Animals, Humans, NAD, Triple Negative Breast Neoplasms, Biosensing Techniques, Niacinamide/metabolism, Pyridinium Compounds, Brain metastases, Cancer prevalence, Human T cells, In vivo noninvasive bioluminescent imaging, NR supplementation, Nicotinamide riboside (NR) uptake
Pubmed
Web of science
Création de la notice
11/11/2022 9:17
Dernière modification de la notice
07/08/2023 9:00
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