Inverse immunostaining pattern for synthesized versus endocytosed alpha-granule proteins in human bone marrow megakaryocytes.

Détails

ID Serval
serval:BIB_C0EC77887D83
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inverse immunostaining pattern for synthesized versus endocytosed alpha-granule proteins in human bone marrow megakaryocytes.
Périodique
British Journal of Haematology
Auteur(s)
de Larouzière V., Brouland J.P., Souni F., Drouet L., Cramer E.
ISSN
0007-1048 (Print)
ISSN-L
0007-1048
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
101
Numéro
4
Pages
618-625
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The time of appearance and pattern of expression of several alpha-granule proteins, von Willebrand factor (VWF), fibrinogen and immunoglobulins (Ig) were examined and compared in human bone marrow megakaryocytes (MK) using an immunocytochemical approach. VWF is synthesized by immature MK, whereas it has been shown that fibrinogen is incorporated from the plasma into alpha-granules. The present study was undertaken in order to determine whether there are chronological and morphological differences in the expression of VWF and fibrinogen in vivo in human MK. Seven paraffin-embedded biopsies of normal human bone marrow were labelled with specific antibodies for VWF and for fibrinogen, detected by the alkaline phosphatase anti-alkaline phosphatase (APAAP) method. and analysed by immunomorphometry. We found a clear, statistically significant. difference in the labelling pattern of VWF and fibrinogen. The expression of other endocytosed alpha-granule proteins, immunoglobulins G and A, was therefore studied in bone marrow MK from two patients with multiple myeloma, one with monoclonal IgG and one with monoclonal IgA. The immunostaining pattern was similar to that of fibrinogen and different from VWF, and characteristic of endocytosed alpha-granule proteins. This study demonstrates that: (i) immunohistochemical staining of MK alpha-granules proteins distinguishes the peripheral cockade distribution pattern of endocytosed protein from the perinuclear pattern of endogenously synthesized proteins; (ii) VWF is present in human bone marrow MK when fibrinogen is not yet detectable: (iii) VWF synthesis ceases while fibrinogen is still being incorporated: (iv) immunoglobulins can be detected in MK cytoplasm, with a staining pattern resembling that of fibrinogen.
Mots-clé
Endocytosis, Fibrinogen/metabolism, Humans, Immunoglobulin A/metabolism, Immunoglobulin G/metabolism, Immunohistochemistry/methods, Megakaryocytes/metabolism, Retrospective Studies, Staining and Labeling/methods, von Willebrand Factor/metabolism
Pubmed
Web of science
Création de la notice
13/10/2015 9:06
Dernière modification de la notice
20/08/2019 15:35
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