ADH-dependent phosphoinositide signalling system and prostaglandin E production in the frog urinary bladder.

Détails

ID Serval
serval:BIB_C09526A895BC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
ADH-dependent phosphoinositide signalling system and prostaglandin E production in the frog urinary bladder.
Périodique
Cellular Signalling
Auteur⸱e⸱s
Parnova R.G., Firsov D.L.
ISSN
0898-6568 (Print)
ISSN-L
0898-6568
Statut éditorial
Publié
Date de publication
1991
Volume
3
Numéro
2
Pages
135-143
Langue
anglais
Résumé
Antidiuretic hormone (ADH) stimulated formation of inositol 1,4,5-trisphosphate (IP3), 1,2-diaclyglycerol (DAG) and an increase of phosphatidylinositol 4,5-biphosphate (PIP2) breakdown in the frog urinary bladder 20 s after addition. ADH also increased the prostaglandin E (PGE) secretion into serosal medium 3.5-fold and the release of arachidonic acid (AA) from 1,2-DAG, which was intensified in the presence of DAG kinase inhibitor R59022. Neomycin sulphate (10(-5) M) from the serosal side blocked ADH-stimulated PIP2 hydrolysis, IP3 production and increased the hydro-osmotic response to ADH. It also inhibited the ADH-stimulated PGE production (55%) and release of AA from 1,2-DAG. This data suggest that PIP2 breakdown is involved in the mechanism of feedback regulation of ADH action and is associated with PGE production via (i) the increase of AA release from PIP2-generated 1,2-DAG and (ii) possible activation of phospholipase A2 by IP3-induced elevation of cytosol Ca2+.
Mots-clé
Animals, Arachidonic Acid, Arachidonic Acids/metabolism, Diglycerides/analysis, Diglycerides/biosynthesis, Inositol 1,4,5-Trisphosphate/biosynthesis, Lipoprotein Lipase/metabolism, Neomycin/pharmacology, Osmosis/drug effects, Phosphatidylinositol 4,5-Diphosphate, Phosphatidylinositols/metabolism, Prostaglandins E/analysis, Prostaglandins E/biosynthesis, Rana temporaria, Signal Transduction/physiology, Type C Phospholipases/antagonists & inhibitors, Urinary Bladder/drug effects, Urinary Bladder/metabolism, Vasopressins/pharmacology, Vasopressins/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 12:32
Dernière modification de la notice
20/08/2019 15:35
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