Role of the V-ATPase in regulation of the vacuolar fission-fusion equilibrium.

Détails

ID Serval
serval:BIB_BF3DFCB1B983
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of the V-ATPase in regulation of the vacuolar fission-fusion equilibrium.
Périodique
Molecular Biology of the Cell
Auteur(s)
Baars T.L., Petri S., Peters C., Mayer A.
ISSN
1059-1524 (Print)
ISSN-L
1059-1524
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
18
Numéro
10
Pages
3873-3882
Langue
anglais
Résumé
Like numerous other eukaryotic organelles, the vacuole of the yeast Saccharomyces cerevisiae undergoes coordinated cycles of membrane fission and fusion in the course of the cell cycle and in adaptation to environmental conditions. Organelle fission and fusion processes must be balanced to ensure organelle integrity. Coordination of vacuole fission and fusion depends on the interactions of vacuolar SNARE proteins and the dynamin-like GTPase Vps1p. Here, we identify a novel factor that impinges on the fusion-fission equilibrium: the vacuolar H(+)-ATPase (V-ATPase) performs two distinct roles in vacuole fission and fusion. Fusion requires the physical presence of the membrane sector of the vacuolar H(+)-ATPase sector, but not its pump activity. Vacuole fission, in contrast, depends on proton translocation by the V-ATPase. Eliminating proton pumping by the V-ATPase either pharmacologically or by conditional or constitutive V-ATPase mutations blocked salt-induced vacuole fragmentation in vivo. In living cells, fission defects are epistatic to fusion defects. Therefore, mutants lacking the V-ATPase display large single vacuoles instead of multiple smaller vacuoles, the phenotype that is generally seen in mutants having defects only in vacuolar fusion. Its dual involvement in vacuole fission and fusion suggests the V-ATPase as a potential regulator of vacuolar morphology and membrane dynamics.
Mots-clé
Biological Transport/drug effects, Epistasis, Genetic, Macrolides/pharmacology, Membrane Fusion/drug effects, Models, Biological, Mutation/genetics, Proton Pumps/antagonists & inhibitors, Protons, Saccharomyces cerevisiae/drug effects, Saccharomyces cerevisiae/enzymology, Vacuolar Proton-Translocating ATPases/metabolism, Vacuoles/drug effects, Vacuoles/enzymology
Pubmed
Web of science
Création de la notice
24/01/2008 15:06
Dernière modification de la notice
20/08/2019 15:33
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