Early detection of human glioma sphere xenografts in mouse brain using diffusion MRI at 14.1 T.

Détails

Ressource 1Télécharger: BIB_BF22F9A4F1B0.P001.pdf (1731.80 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_BF22F9A4F1B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Early detection of human glioma sphere xenografts in mouse brain using diffusion MRI at 14.1 T.
Périodique
NMR in biomedicine
Auteur⸱e⸱s
Porcari P., Hegi M.E., Lei H., Hamou M.F., Vassallo I., Capuani S., Gruetter R., Mlynarik V.
ISSN
1099-1492 (Electronic)
ISSN-L
0952-3480
Statut éditorial
Publié
Date de publication
11/2016
Peer-reviewed
Oui
Volume
29
Numéro
11
Pages
1577-1589
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Glioma models have provided important insights into human brain cancers. Among the investigative tools, MRI has allowed their characterization and diagnosis. In this study, we investigated whether diffusion MRI might be a useful technique for early detection and characterization of slow-growing and diffuse infiltrative gliomas, such as the proposed new models, LN-2669GS and LN-2540GS glioma sphere xenografts. Tumours grown in these models are not visible in conventional T2 -weighted or contrast-enhanced T1 -weighted MRI at 14.1 T. Diffusion-weighted imaging and diffusion tensor imaging protocols were optimized for contrast by exploring long diffusion times sensitive for probing the microstructural alterations induced in the normal brain by the slow infiltration of glioma sphere cells. Compared with T2 -weighted images, tumours were properly identified in their early stage of growth using diffusion MRI, and confirmed by localized proton MR spectroscopy as well as immunohistochemistry. The first evidence of tumour presence was revealed for both glioma sphere xenograft models three months after tumour implantation, while no necrosis, oedema or haemorrhage were detected either by MRI or by histology. Moreover, different values of diffusion indices, such as mean diffusivity and fractional anisotropy, were obtained in tumours grown from LN-2669GS and LN-2540GS glioma sphere lines. These observations highlighted diverse tumour microstructures for both xenograft models, which were reflected in histology. This study demonstrates the ability of diffusion MRI techniques to identify and investigate early stages of slow-growing, invasive tumours in the mouse brain, thus providing a potential imaging biomarker for early detection of tumours in humans.

Mots-clé
Algorithms, Animals, Brain Neoplasms/diagnostic imaging, Brain Neoplasms/pathology, Cell Line, Tumor, Diffusion Magnetic Resonance Imaging/methods, Early Detection of Cancer/methods, Female, Glioma/diagnostic imaging, Glioma/pathology, Humans, Image Enhancement/methods, Image Interpretation, Computer-Assisted/methods, Mice, Mice, Nude, Neoplasm Invasiveness, Reproducibility of Results, Sensitivity and Specificity, Spheroids, Cellular/pathology, ADC, FA, MD, MRS, brain tumours, diffusion MRI, human glioma xenografts, immunohistochemistry
Pubmed
Web of science
Création de la notice
19/10/2016 12:40
Dernière modification de la notice
20/08/2019 15:33
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