Flow pulsatility is a critical determinant of oxidative stress in endothelial cells

Détails

ID Serval
serval:BIB_BF01C005F0AA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Flow pulsatility is a critical determinant of oxidative stress in endothelial cells
Périodique
Hypertension
Auteur⸱e⸱s
Silacci  P., Desgeorges  A., Mazzolai  L., Chambaz  C., Hayoz  D.
ISSN
1524-4563 (Electronic)
Statut éditorial
Publié
Date de publication
11/2001
Volume
38
Numéro
5
Pages
1162-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
Atherosclerotic plaques are found in regions exposed to disturbed flow, suggesting the active participation of the hemodynamic environment in atherogenesis. Indeed, unidirectional and oscillatory flow patterns (ie, bidirectional) have been shown to induce contrasting effects on endothelial function. The purpose of the present study was to evaluate the effect of these 2 flow patterns characterizing plaque-free and plaque-prone regions, respectively, on the oxidative stress of endothelial cells. NADH-dependent oxidase activity was shown to be equally induced (2- to 3-fold) in endothelial cells exposed to pulsatile unidirectional or oscillatory flow patterns. Under these flow conditions, an increase in endothelial cell oxidative state compared with static cultures was observed. Pulsatility of flow, but not cyclic stretch, was a critical determinant of flow-induced superoxide anion production. P22phox mRNA level increased in cells exposed to both unidirectional and oscillatory shear stress, suggesting that p22phox gene expression upregulation contributes to flow-induced increase in superoxide anion production in endothelial cells. In conclusion, we demonstrate a flow-induced increase in oxidative stress in endothelial cells. This chronic increase is dependent on the pulsatile nature of flow and is mediated in part by upregulation of an NADH-dependent oxidase expression.
Mots-clé
Acetylcysteine/pharmacology Animals Antioxidants/pharmacology Arteriosclerosis/metabolism Cattle Cells, Cultured Endothelium, Vascular/drug effects/*physiology Heme Oxygenase (Decyclizing)/metabolism Heme Oxygenase-1 *Membrane Transport Proteins NADPH Dehydrogenase/biosynthesis/genetics NADPH Oxidase Nitric Oxide Synthase/biosynthesis/genetics Nitric Oxide Synthase Type III Oxidation-Reduction *Oxidative Stress Phosphoproteins/biosynthesis/genetics RNA, Messenger/biosynthesis Stress, Mechanical Superoxides/metabolism Up-Regulation/drug effects
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 16:38
Dernière modification de la notice
20/08/2019 15:33
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