Early lymph vessel development from embryonic stem cells.

Détails

ID Serval
serval:BIB_BEDA15A7C04A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Early lymph vessel development from embryonic stem cells.
Périodique
Arteriosclerosis, Thrombosis, and Vascular Biology
Auteur(s)
Kreuger J., Nilsson I., Kerjaschki D., Petrova T., Alitalo K., Claesson-Welsh L.
ISSN
1524-4636 (Electronic)
ISSN-L
1079-5642
Statut éditorial
Publié
Date de publication
2006
Volume
26
Numéro
5
Pages
1073-1078
Langue
anglais
Résumé
OBJECTIVE: The purpose of this study was to establish a model system for lymph vessel development based on directed differentiation of murine embryonic stem cells.
METHODS AND RESULTS: Stem cells were aggregated to form embryoid bodies, and subsequently cultured in 3-dimensional collagen matrix for up to 18 days. Treatment with vascular endothelial growth factor (VEGF)-C and VEGF-A individually enhanced formation of lymphatic vessel structures, although combined treatment with VEGF-C and VEGF-A was most potent and gave rise to a network of LYVE-1, podoplanin, Prox1, and VEGF receptor-3 positive lymphatic vessel structures running parallel to and apparently emanating from, capillaries. In contrast, fibroblast growth factor-2, hepatocyte growth factor, or hypoxia had little or no effect on the development of the early lymphatics. Further, cells of hematopoietic origin were shown to express lymphatic markers. In summary, different subpopulations of lymphatic endothelial cells were identified on the basis of differential expression of several lymphatic and blood vessel markers, indicating vascular heterogeneity.
CONCLUSIONS: We conclude that the present model closely mimics the early steps of lymph vessel development in mouse embryos.
Mots-clé
Animals, Antigens, CD31/analysis, Cell Differentiation, Cell Hypoxia, Cells, Cultured, Embryo, Mammalian/cytology, Fibroblast Growth Factor 2/pharmacology, Glycoproteins/analysis, Glycoproteins/physiology, Hepatocyte Growth Factor/pharmacology, Homeodomain Proteins/analysis, Lymphangiogenesis, Mice, Stem Cells/cytology, Tumor Suppressor Proteins, Vascular Endothelial Growth Factor A/pharmacology, Vascular Endothelial Growth Factor C/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/12/2012 17:10
Dernière modification de la notice
20/08/2019 16:33
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