Tropomyosin-4 correlates with higher SBR grades and tubular differentiation in infiltrating ductal breast carcinomas: an immunohistochemical and proteomics-based study.
Détails
ID Serval
serval:BIB_BE82349FA2F8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tropomyosin-4 correlates with higher SBR grades and tubular differentiation in infiltrating ductal breast carcinomas: an immunohistochemical and proteomics-based study.
Périodique
Tumour biology
ISSN
1423-0380 (Electronic)
ISSN-L
1010-4283
Statut éditorial
Publié
Date de publication
12/2013
Peer-reviewed
Oui
Volume
34
Numéro
6
Pages
3593-3602
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The aim of this study is to evaluate tropomyosin-4 (TM4) expression in infiltrating ductal breast carcinomas (IDCAs), as well as its prognostic significance. Using a 2-DE/MALDI-TOF mass spectrometry investigation coupled with an immunohistochemical approach, we have assessed the expression of TM4 in IDCAs, as well as in other types of breast tumors. Proteomic analyses revealed an increased expression of tropomyosin-4 in IDCA tumors. Using immunohistochemistry, overexpression of tropomyosin-4 was confirmed in 51 additional tumor specimens. Statistical analyses revealed, however, no significant correlations between tropomyosin-4 expression and clinicopathological parameters of the disease including tumor stage, patient age, estrogen and progesterone receptor status, and lymph node metastasis occurrence. A significant association was found, however, with a high Scarf-Bloom-Richardson (SBR) grade, a known marker of tumor severity. Additionally, the SBR component showing a correlation with TM4 expression was the tubular differentiation status. This study demonstrates the upregulation of tropomyosin-4 in IDCA tissues, which may highlight its involvement in breast cancer development. Our findings also support a link between tropomyosin-4 expression and aggressiveness of IDCA tumors.
Mots-clé
Adult, Amino Acid Sequence, Blotting, Western, Breast/metabolism, Breast/pathology, Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Carcinoma, Ductal, Breast/metabolism, Carcinoma, Ductal, Breast/pathology, Electrophoresis, Gel, Two-Dimensional, Female, Humans, Immunohistochemistry, Lymphatic Metastasis, Molecular Sequence Data, Neoplasm Grading, Neoplasm Staging, Proteome/metabolism, Proteomics/methods, Severity of Illness Index, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tropomyosin/metabolism, Up-Regulation
Pubmed
Web of science
Création de la notice
17/10/2023 8:03
Dernière modification de la notice
20/10/2023 6:10