Versatility of Pyridoxal Phosphate as a Coating of Iron Oxide Nanoparticles.

Détails

Ressource 1Télécharger: nanomaterials-07-00202.pdf (3364.61 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_BE46CDFFBA65
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Versatility of Pyridoxal Phosphate as a Coating of Iron Oxide Nanoparticles.
Périodique
Nanomaterials
Auteur(s)
Bonvin D., Aschauer U.J., Bastiaansen JAM, Stuber M., Hofmann H., Mionić Ebersold M.
ISSN
2079-4991 (Print)
ISSN-L
2079-4991
Statut éditorial
Publié
Date de publication
29/07/2017
Peer-reviewed
Oui
Volume
7
Numéro
8
Pages
202
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Pyridoxal 5'-phosphate (PLP) is the most important cofactor of vitamin B₆-dependent enzymes, which catalyses a wide range of essential body functions (e.g., metabolism) that could be exploited to specifically target highly metabolic cells, such as tumour metastatic cells. However, the use of PLP as a simultaneous coating and targeting molecule, which at once provides colloidal stability and specific biological effects has not been exploited so far. Therefore, in this work iron oxide nanoparticles (IONPs) were coated by PLP at two different pH values to tune PLP bonding (e.g., orientation) at the IONP surface. The surface study, as well as calculations, confirmed different PLP bonding to the IONP surface at these two pH values. Moreover, the obtained PLP-IONPs showed different zeta potential, hydrodynamic radius and agglomeration state, and consequently different uptake by two metastatic-prostate-cancer cell lines (LnCaP and PC3). In LnCaP cells, PLP modified the morphology of IONP-containing intracellular vesicles, while in PC3 cells PLP impacted the amount of IONPs taken up by cells. Moreover, PLP-IONPs displayed high magnetic resonance imaging (MRI) r₂ relaxivity and were not toxic for the two studied cell lines, rendering PLP promising for biomedical applications. We here report the use of PLP simultaneously as a coating and targeting molecule, directly bound to the IONP surface, with the additional high potential for MRI detection.

Mots-clé
cellular uptake, iron oxide nanoparticles, magnetic resonance imaging, pyridoxal 5′-phosphate, surface functionalization
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/08/2017 12:15
Dernière modification de la notice
20/08/2019 16:32
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