Formation and inactivation of endogenous cannabinoid anandamide in central neurons

Détails

ID Serval
serval:BIB_BE2D2AFBC177
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Formation and inactivation of endogenous cannabinoid anandamide in central neurons
Périodique
Nature
Auteur⸱e⸱s
Di Marzo V., Fontana A., Cadas H., Schinelli S., Cimino G., Schwartz J. C., Piomelli D.
ISSN
0028-0836 (Print)
ISSN-L
0028-0836
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
372
Numéro
6507
Pages
686-91
Langue
anglais
Notes
Di Marzo, V
Fontana, A
Cadas, H
Schinelli, S
Cimino, G
Schwartz, J C
Piomelli, D
eng
Research Support, Non-U.S. Gov't
England
Nature. 1994 Dec 15;372(6507):686-91.
Résumé
Anandamide (N-arachidonoyl-ethanolamine) was recently identified as a brain arachidonate derivative that binds to and activates cannabinoid receptors, yet the mechanisms underlying formation, release and inactivation of this putative messenger molecule are still unclear. Here we report that anandamide is produced in and released from cultured brain neurons in a calcium ion-dependent manner when the neurons are stimulated with membrane-depolarizing agents. Anandamide formation occurs through phosphodiesterase-mediated cleavage of a novel phospholipid precursor, N-arachidonoyl-phosphatidylethanolamine. A similar mechanism also governs the formation of a family of anandamide congeners, whose possible roles in neuronal signalling remain unknown. Our results and those of others indicate therefore that multiple biochemical pathways may participate in anandamide formation in brain tissue. The life span of extracellular anandamide is limited by a rapid and selective process of cellular uptake, which is accompanied by hydrolytic degradation to ethanolamine and arachidonate. Our results thus strongly support the proposed role of anandamide as an endogenous neuronal messenger.
Mots-clé
Animals, Arachidonic Acids/*metabolism, Astrocytes/metabolism, Cannabinoids/*metabolism, Cells, Cultured, Central Nervous System/drug effects/*metabolism, Cerebral Cortex/metabolism, Corpus Striatum/metabolism, Endocannabinoids, Ionomycin/pharmacology, Neurons/drug effects/*metabolism, Phosphatidylethanolamines/metabolism, Polyunsaturated Alkamides, Rats, *Signal Transduction
Pubmed
Création de la notice
13/11/2017 9:57
Dernière modification de la notice
20/08/2019 15:32
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